Challenges and progress towards industrial recombinant protein production in yeasts: a review

P De Brabander, E Uitterhaegen, T Delmulle… - Biotechnology …, 2023 - Elsevier
Recombinant proteins (RP) are widely used as biopharmaceuticals, industrial enzymes, or
sustainable food source. Yeasts, with their ability to produce complex proteins through a …

[HTML][HTML] Flow cytometry for the development of biotechnological processes with microalgae

P Hyka, S Lickova, P Přibyl, K Melzoch, K Kovar - Biotechnology advances, 2013 - Elsevier
The current interest in microalgae as a sustainable source of next generation biofuels and
other valuable substances is driving exploration of their use as unique biotechnological …

Bioprocess engineering aspects of heterologous protein production in Pichia pastoris: a review

G Potvin, A Ahmad, Z Zhang - Biochemical Engineering Journal, 2012 - Elsevier
Pichia pastoris is currently one of the most effective and versatile systems for the expression
of heterologous proteins. Its success is due to its powerful methanol-inducible alcohol …

Advances in recombinant lipases: Production, engineering, immobilization and application in the pharmaceutical industry

FJ Contesini, MG Davanço, GP Borin, KG Vanegas… - Catalysts, 2020 - mdpi.com
Lipases are one of the most used enzymes in the pharmaceutical industry due to their
efficiency in organic syntheses, mainly in the production of enantiopure drugs. From an …

Systems biotechnology for protein production in Pichia pastoris

RJ Zahrl, DA Peña, D Mattanovich… - FEMS yeast …, 2017 - academic.oup.com
The methylotrophic yeast Pichia pastoris (syn. Komagataella spp.) is one of the most
important production systems for heterologous proteins. After the first genome sequences …

Recombinant protein production in yeasts

D Porro, M Sauer, P Branduardi, D Mattanovich - Molecular biotechnology, 2005 - Springer
Recombinant DNA (rDNA) technologies (genetic, protein, and metabolic engineering) allow
the production of a wide range of peptides, proteins, and biochemicals from naturally …

Stress in recombinant protein producing yeasts

D Mattanovich, B Gasser, H Hohenblum… - Journal of biotechnology, 2004 - Elsevier
It is well established today that heterologous overexpression of proteins is connected with
different stress reactions. The expression of a foreign protein at a high level may either …

Genome, secretome and glucose transport highlight unique features of the protein production host Pichia pastoris

D Mattanovich, A Graf, J Stadlmann, M Dragosits… - Microbial cell …, 2009 - Springer
Background Pichia pastoris is widely used as a production platform for heterologous
proteins and model organism for organelle proliferation. Without a published genome …

The Effect of Temperature on the Proteome of Recombinant Pichia pastoris

M Dragosits, J Stadlmann, J Albiol… - Journal of proteome …, 2009 - ACS Publications
The impact of environmental factors on the productivity of yeast cells is poorly investigated
so far. Therefore, it is a major concern to improve the understanding of cellular physiology of …

Production of Industrial Enzymes via Pichia pastoris as a Cell Factory in Bioreactor: Current Status and Future Aspects

ZE Duman-Özdamar, B Binay - The protein journal, 2021 - Springer
Industrial enzymes have been widely preferred in various industries such as chemical
production, food & beverage, pharmaceutical, textile, cosmetics, etc. due to the …