Approaches and materials for endocytosis-independent intracellular delivery of proteins

N Chen, Y He, M Zang, Y Zhang, H Lu, Q Zhao… - Biomaterials, 2022 - Elsevier
The intracellular delivery of proteins is of great significance. For diseases such as cancer,
heart disease and neurodegenerative diseases, many important pharmacological targets …

Arginine-rich cell-penetrating peptides induce membrane multilamellarity and subsequently enter via formation of a fusion pore

C Allolio, A Magarkar, P Jurkiewicz… - Proceedings of the …, 2018 - National Acad Sciences
Arginine-rich cell-penetrating peptides do not enter cells by directly passing through a lipid
membrane; they instead passively enter vesicles and live cells by inducing membrane …

Nonuniversal impact of cholesterol on membranes mobility, curvature sensing and elasticity

M Pöhnl, MFW Trollmann, RA Böckmann - Nature Communications, 2023 - nature.com
Biological membranes, composed mainly of phospholipids and cholesterol, play a vital role
as cellular barriers. They undergo localized reshaping in response to environmental cues …

Pre-existing bilayer stresses modulate triglyceride accumulation in the ER versus lipid droplets

V Zoni, R Khaddaj, P Campomanes, AR Thiam… - Elife, 2021 - elifesciences.org
Cells store energy in the form of neutral lipids (NLs) packaged into micrometer-sized
organelles named lipid droplets (LDs). These structures emerge from the endoplasmic …

[HTML][HTML] Modeling coupled single cell electroporation and thermal effects from nanosecond electric pulse trains

W Milestone, Q Hu, AM Loveless, AL Garner… - Journal of Applied …, 2022 - pubs.aip.org
A distributed circuit approach is used to simulate the development of electric potentials
across a cell membrane and the resulting poration dynamics for∼ 700 ns duration voltage …

Calcium ions promote membrane fusion by forming negative-curvature inducing clusters on specific anionic lipids

C Allolio, D Harries - ACS nano, 2021 - ACS Publications
Vesicles enriched in certain negatively charged lipids, such as phosphatidylserine and PIP2,
are known to undergo fusion in the presence of calcium ions without assistance from protein …

Lipid bicelles in the study of biomembrane characteristics

M Pöhnl, C Kluge, RA Böckmann - Journal of Chemical Theory …, 2023 - ACS Publications
Simulations of lipid membranes typically make use of periodic boundary conditions to mimic
macroscopically sized membranes and allow for comparison to experiments performed eg …

Measuring the bending modulus of lipid bilayers with cholesterol

JF Nagle - Physical Review E, 2021 - APS
The effect of cholesterol on the bending modulus of DOPC lipid bilayers has become a
controversial topic that has implications for methods of measuring the bending modulus …

Confinement in nanodiscs anisotropically modifies lipid bilayer elastic properties

I Schachter, C Allolio, G Khelashvili… - The Journal of Physical …, 2020 - ACS Publications
Lipid nanodiscs are small synthetic lipid bilayer structures that are stabilized in solution by
special circumscribing (or scaffolding) proteins or polymers. Because they create native-like …

Elastic moduli of normal and cancer cell membranes revealed by molecular dynamics simulations

HL Nguyen, VH Man, MS Li, P Derreumaux… - Physical Chemistry …, 2022 - pubs.rsc.org
Recent studies indicate that there are mechanical differences between normal cells and
cancer cells. Because the cell membrane takes part in a variety of vital processes, we test …