Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact
tissues from individual organs and even to some entire mammals. When combined with light …

The cerebral vasculature is a dense network of arteries, capillaries, and veins. Quantifying
variations of the vascular organization across individuals, brain regions, or disease models …

Neuronal cell types are the nodes of neural circuits that determine the flow of information
within the brain. Neuronal morphology, especially the shape of the axonal arbor, provides …

INTRODUCTION Neural circuits across the brain are composed of structures spanning
seven orders of magnitude in size that are assembled from thousands of distinct protein …

Spinal cord injury (SCI) induces haemodynamic instability that threatens survival,–, impairs
neurological recovery,, increases the risk of cardiovascular disease,, and reduces quality of …

Recent progress in understanding the diversity of midbrain dopamine neurons has
highlighted the importance—and the challenges—of defining mammalian neuronal cell …

Whole-brain mesoscale mapping in primates has been hindered by large brain sizes and
the relatively low throughput of available microscopy methods. Here, we present an …

CLARITY is a method for chemical transformation of intact biological tissues into a hydrogel-
tissue hybrid, which becomes amenable to interrogation with light and macromolecular …

Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that
consist of many unaligned three-dimensional image tiles, which must be reconstructed …