Hydrophobic interaction chromatography of proteins

JA Queiroz, CT Tomaz, JMS Cabral - Journal of biotechnology, 2001 - Elsevier
In this article, an overview of hydrophobic interaction chromatography (HIC) of proteins is
given. After a brief description of protein hydrophobicity and hydrophobic interactions, we …

Aqueous two-phase systems for biomolecule separation

AD Diamond, JT Hsu - Bioseparation, 2006 - Springer
Over the past-thirty years, aqueous polymer two-phase technology has evolved, both
experimentally and theoretically, into a separation science with many useful applications in …

[图书][B] Enzymes in industry: production and applications

W Aehle - 2007 - books.google.com
Leading experts from all over the world present an overview of the use of enzymes in
industry for:-the production of bulk products, such as glucose, or fructose-food processing …

Substrate gradient formation in the large-scale bioreactor lowers cell yield and increases by-product formation

F Bylund, E Collet, SO Enfors, G Larsson - Bioprocess Engineering, 1998 - Springer
A heterogeneous micro-environment was identified in a 12 m 3 bioreactor with a height-to-
diameter ratio of 2.5. The reactor was aerated by a ring sparger and stirred by three Rushton …

Purification of papain from Carica papaya latex: Aqueous two-phase extraction versus two-step salt precipitation

S Nitsawang, R Hatti-Kaul, P Kanasawud - Enzyme and Microbial …, 2006 - Elsevier
Purification of papain from wet Carica papaya latex by extraction in aqueous two-phase
system was studied and compared with the traditional procedure involving a two-step salt …

A new excluded volume theory and its application to the coexistence curves of aqueous polymer two-phase systems

Y Guan, TH Lilley, TE Treffry - Macromolecules, 1993 - ACS Publications
A theoretical description of the coexistence curves of binary and some ternary systems has
been developed using methods of statistical geometry. It is based on the concept that …

[图书][B] Enzymes in industry: production and applications.

W Gerhartz - 1990 - cabidigitallibrary.org
Industrial uses of enzymes are discussed under the following chapter headings: introduction
(history, enzyme nomenclature and structure, and molecular biology); catalytic activity of …

Theoretical Aspects and Applications of Aqueous Two‐Phase Systems

M Singla, N Sit - ChemBioEng Reviews, 2023 - Wiley Online Library
The aqueous two‐phase system (ATPS) is commonly known as a technique that yields high‐
purity products in a single step. It is particularly advantageous for purifying biomolecules like …

Bioconversions in aqueous two-phase systems

E Andersson, B Hahn-Hägerdal - Enzyme and microbial technology, 1990 - Elsevier
Bioconversions involving enzymes and/or microbial cells in aqueous two-phase systems are
reviewed. The partitioning of biocatalysts, substrates, and products is discussed in relation to …

Aqueous two-phase poly (ethylene glycol)–poly (acrylic acid) system for protein partitioning: Influence of molecular weight, pH and temperature

S Saravanan, JR Rao, BU Nair, T Ramasami - Process biochemistry, 2008 - Elsevier
The partitioning behavior of two model proteins in poly (ethylene glycol)(PEG)–poly (acrylic
acid)(PAA) aqueous two-phase systems has been studied. The effect of polymer molecular …