The temperature-dependent characteristics of fluorescence of several rare-earth-doped
ceramic phosphors has made these materials the focus of a major effort in the field of …

Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide
information, not only concerning the localisation of specific fluorophores, but also about the …

Fluorescence lifetime images are obtained with the laser scanning microscope using two
methods: the time-correlated single-photon counting method and the frequency-domain …

Two-photon fluorescence lifetime imaging is used to identify microdomains (1–25μm) of two
distinct pH values within the uppermost layer of the epidermis (stratum corneum). The …

The fluorescence lifetime value of tryptophan residues varies by more than a factor of 100 in
different proteins and is determined by several factors, which include solvent exposure and …

Although the phenomenon of molecular fluorescence emission contains both spectral
(typically UV/visible) and time (typically 10–9 s) information, it is the former which has …

The decay of the tryptophanyl emission in proteins is often complex due to the sensitivity of
the tryptophan excited state to its surroundings. The traditional analysis of the decay curve …

The analysis of the fluorescence decay using discrete exponential components assumes
that a small number of species is present. In the absence of a definite kinetic model or when …

A novel technique for suppression of the multi-photon fluorescence contribution in second-
order nonlinear optical hyper-Rayleigh scattering experiments is described. The technique …

We hope that we have conveyed information of interest and value to present and future
fluorescence practitioners. Those readers with a sustaining interest in this topic may wish to …