The contributions of plasmids to biology and their impact in biotechnology and discovery have been immense. Together with restriction enzymes, plasmids were one of the key …
I Perçin, E Sağlar, H Yavuz, E Aksöz… - International journal of …, 2011 - Elsevier
The aim of this study is to prepare supermacroporous pseudospecific cryogel which can be used for the purification of plasmid DNA (pDNA) from bacterial lysate. N-methacryloyl-(l) …
The aim of this study is to prepare poly (hydroxyethyl methacrylate-N-methacryloyl-(L)- histidine)[PHEMAH] magnetic nanoparticles for plasmid DNA (pDNA) purification from …
In negative chromatography, the impurities bind on the adsorbent, and the product is allowed to flow through the chromatographic column. Negative chromatography is an …
The aim of this study is to prepare megaporous dye-affinity cryogel discs for the purification of plasmid DNA (pDNA) from bacterial lysate. Poly (hydroxyethyl methacrylate)[PHEMA] …
Non-viral gene therapy and DNA vaccination currently hold great potential for treatment and prevention of genetic and acquired diseases. The large-scale manufacturing of plasmid …
This work describes a process for the purification of minicircles (MC), obtained from Escherichia coli by intramolecular recombination of a parental plasmid (PP) into a target MC …
AM Azevedo, AG Gomes, L Borlido… - Journal of Molecular …, 2010 - Wiley Online Library
In this work, we investigated the feasibility of using phenyl boronate (PB) chromatography for the direct capture of monoclonal antibodies from a CHO cell supernatant. Preliminary …
In this work, we systematically evaluated the potential of using boronic acid functionalized magnetic particles in the capturing of human immunoglobulin G under typical mammalian …