[HTML][HTML] Recent advances in chromatographic purification of plasmid DNA for gene therapy and DNA vaccines: A review

A Abdulrahman, A Ghanem - Analytica chimica acta, 2018 - Elsevier
The wide spread of infectious diseases have provoked the scientists to develop new types of
vaccines. Among the different types of vaccines, the recently discovered plasmid DNA …

Plasmid biopharmaceuticals

DMF Prazeres, GA Monteiro - Plasmids: Biology and impact in …, 2015 - Wiley Online Library
The contributions of plasmids to biology and their impact in biotechnology and discovery
have been immense. Together with restriction enzymes, plasmids were one of the key …

Poly (hydroxyethyl methacrylate) based affinity cryogel for plasmid DNA purification

I Perçin, E Sağlar, H Yavuz, E Aksöz… - International journal of …, 2011 - Elsevier
The aim of this study is to prepare supermacroporous pseudospecific cryogel which can be
used for the purification of plasmid DNA (pDNA) from bacterial lysate. N-methacryloyl-(l) …

Poly (hydroxyethyl methacrylate) based magnetic nanoparticles for plasmid DNA purification from Escherichia coli lysate

I Perçin, V Karakoç, S Akgöl, E Aksöz… - Materials Science and …, 2012 - Elsevier
The aim of this study is to prepare poly (hydroxyethyl methacrylate-N-methacryloyl-(L)-
histidine)[PHEMAH] magnetic nanoparticles for plasmid DNA (pDNA) purification from …

Negative chromatography: Progress, applications and future perspectives

MFX Lee, ES Chan, BT Tey - Process Biochemistry, 2014 - Elsevier
In negative chromatography, the impurities bind on the adsorbent, and the product is
allowed to flow through the chromatographic column. Negative chromatography is an …

Dye affinity cryogels for plasmid DNA purification

D Çimen, F Yılmaz, I Perçin, D Türkmen… - Materials Science and …, 2015 - Elsevier
The aim of this study is to prepare megaporous dye-affinity cryogel discs for the purification
of plasmid DNA (pDNA) from bacterial lysate. Poly (hydroxyethyl methacrylate)[PHEMA] …

A process for supercoiled plasmid DNA purification based on multimodal chromatography

AR Silva-Santos, CPA Alves, DMF Prazeres… - Separation and …, 2017 - Elsevier
Non-viral gene therapy and DNA vaccination currently hold great potential for treatment and
prevention of genetic and acquired diseases. The large-scale manufacturing of plasmid …

Multimodal chromatography of supercoiled minicircles: A closer look into DNA-ligand interactions

AR Silva-Santos, CPA Alves, G Monteiro… - Separation and …, 2019 - Elsevier
This work describes a process for the purification of minicircles (MC), obtained from
Escherichia coli by intramolecular recombination of a parental plasmid (PP) into a target MC …

Capture of human monoclonal antibodies from a clarified cell culture supernatant by phenyl boronate chromatography

AM Azevedo, AG Gomes, L Borlido… - Journal of Molecular …, 2010 - Wiley Online Library
In this work, we investigated the feasibility of using phenyl boronate (PB) chromatography for
the direct capture of monoclonal antibodies from a CHO cell supernatant. Preliminary …

Potential of boronic acid functionalized magnetic particles in the adsorption of human antibodies under mammalian cell culture conditions

L Borlido, AM Azevedo, ACA Roque… - … of Chromatography A, 2011 - Elsevier
In this work, we systematically evaluated the potential of using boronic acid functionalized
magnetic particles in the capturing of human immunoglobulin G under typical mammalian …