Bioorthogonal chemistry is a set of methods using the chemistry of non-native functional
groups to explore and understand biology in living organisms. In this review, we summarize …

The last three decades have brought a revolution in fluorescence microscopy. The
development of new microscopes, fluorescent labels and analysis techniques has pushed …

Bioorthogonal chemistry represents a class of high-yielding chemical reactions that proceed
rapidly and selectively in biological environments without side reactions towards …

Click chemistry is an immensely powerful technique for the fast and efficient covalent
conjugation of molecular entities. Its broad scope has positively impacted on multiple …

Over 20 years ago, the pyrrolysine encoding translation system was discovered in specific
archaea. Our Review provides an overview of how the once obscure pyrrolysyl-tRNA …

Glycans are involved in numerous biological recognition events. Being secondary gene
products, their labeling by genetic methods–comparable to GFP labeling of proteins–is not …

Bioorthogonal chemistry is a powerful tool to site-specifically activate drugs in living systems.
Bioorthogonal reactions between a pair of biologically reactive groups can rapidly and …

The emerging inverse electron demand Diels–Alder (IEDDA) reaction stands out from other
bioorthogonal reactions by virtue of its unmatchable kinetics, excellent orthogonality and …

Bioorthogonal reactions have found widespread use in applications ranging from glycan
engineering to in vivo imaging. Researchers have devised numerous reactions that can be …

Bioorthogonal pretargeting optical imaging shows the potential for enhanced diagnosis and
prognosis. However, the bioorthogonal handles, known for being “always reactive”, may …