Metabolomics aims at identification and quantitation of small molecules involved in
metabolic reactions. LC-MS has enjoyed a growing popularity as the platform for …

Corynebacterium glutamicum, Escherichia coli, and Saccharomyces cerevisiae in particular,
have become established as important industrial workhorses in biotechnology. Recent years …

Direct injection of samples on high-resolving mass spectrometers is an effective way to
maximize analytical throughput and yet allow analyte discrimination in complex samples by …

The field of metabolomics continues to grow rapidly over the last decade and has been
proven to be a powerful technology in predicting and explaining complex phenotypes in …

Overflow metabolism is well known for yeast, bacteria and mammalian cells. It typically
occurs under glucose excess conditions and is characterized by excretions of by-products …

We present a comprehensive overview of the hierarchical network of intracellular processes
revolving around central nitrogen metabolism in Escherichia coli. The hierarchy intertwines …

Quantification of metabolites is of pivotal relevance in biology, where it complements more
established omics techniques such as transcriptomics and proteomics. Here, we present a …

Accurate determination of intracellular metabolite levels requires well-validated procedures
for sampling and sample treatment. Several methods exist for metabolite extraction, but the …

Microbes face a trade-off between being metabolically independent and relying on
neighboring organisms for the supply of some essential metabolites. This balance of …

Acetogens are promising cell factories for producing fuels and chemicals from waste
feedstocks via gas fermentation, but quantitative characterization of carbon, energy, and …