Advances and challenges in biotechnological production of chondroitin sulfate and its oligosaccharides

W Zhang, R Xu, J Chen, H Xiong, Y Wang… - International journal of …, 2023 - Elsevier
Chondroitin sulfate (CS) is a member of glycosaminoglycans (GAGs) and has critical
physiological functions. CS is widely applied in medical and clinical fields. Currently, the …

Hungatella hathewayi, an Efficient Glycosaminoglycan-Degrading Firmicutes from Human Gut and Its Chondroitin ABC Exolyase with High Activity and Broad …

PS Rawat, Y Li, W Zhang, X Meng… - Applied and …, 2022 - Am Soc Microbiol
The degradation of glycosaminoglycans (GAGs) by intestinal bacteria is critical for their
colonization in the human gut and the health of the host. Both colonic Bacteroides and …

Characterization of a Thermostable and Surfactant-Tolerant Chondroitinase B from a Marine Bacterium Microbulbifer sp. ALW1

M Mou, Q Hu, H Li, L Long, Z Li, X Du, Z Jiang… - International Journal of …, 2022 - mdpi.com
Chondroitinase plays an important role in structural and functional studies of chondroitin
sulfate (CS). In this study, a new member of chondroitinase B of PL6 family, namely ChSase …

Identification and characterization of a novel heparinase PCHepII from marine bacterium Puteibacter caeruleilacunae

D Lu, L Wang, Z Ning, Z Li, M Li, Y Jia, Q Zhang - Scientific Reports, 2023 - nature.com
Heparin (HP) and heparan sulfate (HS) are multifunctional polysaccharides widely used in
clinical therapy. Heparinases (Hepases) are enzymes that specifically catalyse HP and HS …

[HTML][HTML] Engineering of Substrate-Binding Domain to Improve Catalytic Activity of Chondroitin B Lyase with Semi-Rational Design

M Tian, YY Xu, YN Li, S Yu, YL Wang, XL Ma… - Current Issues in …, 2024 - mdpi.com
Dermatan sulfate and chondroitin sulfate are dietary supplements that can be utilized as
prophylactics against thrombus formation. Low-molecular-weight dermatan sulfate (LMWDS) …

Identification and characterization of a chondroitinase ABC with a novel carbohydrate-binding module

G Liu, L Song, J Li, X Song, X Mei, Y Zhang… - International Journal of …, 2024 - Elsevier
Chondroitinases play important roles in structural and functional studies of chondroitin
sulfates. Carbohydrate-binding module (CBM) is generally considered as an accessory …

Identification and characterization of a PL35 GAGs lyase with 4-O-sulfated N-acetylgalactosamine (A-type)-rich structures producing property

D Lu, W Wang, X Li, L Wang, Y Guo, C Zhu… - International Journal of …, 2024 - Elsevier
Glycosaminoglycan (GAG) lyases are important tools for investigating the structure of GAGs
and preparing low-molecular-weight GAGs. The PL35 family, a recently established …

Identification and Action Patterns of Two Chondroitin Sulfate Sulfatases From a Marine Bacterium Photobacterium sp. QA16

L Wei, Q Zhang, D Lu, M Du, X Xu, W Wang… - Frontiers in …, 2022 - frontiersin.org
Chondroitin sulfate (CS)/dermatan sulfate (DS) is a kind of sulfated polyanionic, linear
polysaccharide belonging to glycosaminoglycan. CS/DS sulfatases, which specifically …

Enzymatic comparison of two homologous enzymes reveals N-terminal domain of chondroitinase ABC I regulates substrate selection and product generation

M Du, L Wei, M Yuan, R Zou, Y Xu, X Wang… - Journal of Biological …, 2023 - ASBMB
Chondroitinase ABC-type I (CSase ABC I), which can digest both chondroitin sulfate (CS)
and dermatan sulfate (DS) in an endolytic manner, is an essential tool in structural and …

[HTML][HTML] Discovery of a class of glycosaminoglycan lyases with ultrabroad substrate spectrum and their substrate structure preferences

L Wei, R Zou, M Du, Q Zhang, D Lu, Y Xu, X Xu… - Journal of Biological …, 2024 - Elsevier
Glycosaminoglycan (GAG) lyases are often strictly substrate specific, and it is especially
difficult to simultaneously degrade GAGs with different types of glycosidic bonds. Herein, we …