The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology
Harnessing the process of natural selection to obtain and understand new microbial
phenotypes has become increasingly possible due to advances in culturing techniques …
phenotypes has become increasingly possible due to advances in culturing techniques …
Escherichia coli as a host for metabolic engineering
Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
Adaptive laboratory evolution–principles and applications for biotechnology
M Dragosits, D Mattanovich - Microbial cell factories, 2013 - Springer
Adaptive laboratory evolution is a frequent method in biological studies to gain insights into
the basic mechanisms of molecular evolution and adaptive changes that accumulate in …
the basic mechanisms of molecular evolution and adaptive changes that accumulate in …
The molecular diversity of adaptive convergence
O Tenaillon, A Rodríguez-Verdugo, RL Gaut… - Science, 2012 - science.org
To estimate the number and diversity of beneficial mutations, we experimentally evolved 115
populations of Escherichia coli to 42.2° C for 2000 generations and sequenced one genome …
populations of Escherichia coli to 42.2° C for 2000 generations and sequenced one genome …
Exploiting nongenetic cell-to-cell variation for enhanced biosynthesis
Biosynthesis enables renewable production of manifold compounds, yet often biosynthetic
performance must be improved for it to be economically feasible. Nongenetic, cell-to-cell …
performance must be improved for it to be economically feasible. Nongenetic, cell-to-cell …
Rapid evolution of silver nanoparticle resistance in Escherichia coli
JL Graves Jr, M Tajkarimi, Q Cunningham… - Frontiers in …, 2015 - frontiersin.org
The recent exponential increase in the use of engineered nanoparticles (eNPs) means both
greater intentional and unintentional exposure of eNPs to microbes. Intentional use includes …
greater intentional and unintentional exposure of eNPs to microbes. Intentional use includes …
Elucidating the molecular architecture of adaptation via evolve and resequence experiments
Evolve and resequence (E&R) experiments use experimental evolution to adapt populations
to a novel environment, then next-generation sequencing to analyse genetic changes. They …
to a novel environment, then next-generation sequencing to analyse genetic changes. They …
Use of adaptive laboratory evolution to discover key mutations enabling rapid growth of Escherichia coli K-12 MG1655 on glucose minimal medium
RA LaCroix, TE Sandberg, EJ O'Brien… - Applied and …, 2015 - Am Soc Microbiol
Adaptive laboratory evolution (ALE) has emerged as an effective tool for scientific discovery
and addressing biotechnological needs. Much of ALE's utility is derived from reproducibly …
and addressing biotechnological needs. Much of ALE's utility is derived from reproducibly …
Adaptive laboratory evolution—harnessing the power of biology for metabolic engineering
Adaptive laboratory evolution (ALE) strategies allow for the metabolic engineering of
microorganisms by combining genetic variation with the selection of beneficial mutations in …
microorganisms by combining genetic variation with the selection of beneficial mutations in …
Microbial laboratory evolution in the era of genome‐scale science
Laboratory evolution studies provide fundamental biological insight through direct
observation of the evolution process. They not only enable testing of evolutionary theory and …
observation of the evolution process. They not only enable testing of evolutionary theory and …