The plasmid pBR322 was one of the first EK2 multipurpose cloning vectors to be designed
and constructed (ten years ago) for the efficient cloning and selection of recombinant DNA …

* Corresponding author. t Present address: Biotechnology Australia Pty. Ltd., PO Box 20,
Roseville, NSW 2069, Australia. separation of the two plasmid DNA strands, strand transfer …

A cDNA coding for human interleukin-2 (IL-2) has been cloned from a cDNA library prepared
from partially purified IL-2 mRNA. The DNA sequence codes for a polypeptide which …

We examined the promoter activity of the 1.3-kb chicken β-actin gene sequence located
between the 5'flanking region and the proximal region of the second exon. This promoter …

Summary A 2.6 kb plasmid, named pBBR1, was isolated from Bordetella bronchiseptica
S87. After insertion of an antibiotic resistance marker, this plasmid could be transferred into …

Yeast is one of the oldest domesticated organisms and has both industrial and domestic
applications. In addition, it is very widely used as a eukaryotic model organism in biological …

We have constructed phage λ and plasmid DNA substrates (λ tk 2 and p tk 2) that contain
two defective herpesvirus thymidine kinase (tk) genes that can be used to detect …

We have previously described several helper independent vector constructions (S. Hughes
and E. Kosik, Virology 136: 89-99, 1984; J. Sorge and SH Hughes, J. Mol. Appl. Genet. 1 …

When the cells or tissues of most eukaryotes are exposed to elevated temperatures, they
respond with the vigorous induction of a small number of 'heat shock'proteins (hsps). I report …

Only two avian oncogenic viruses specifically cause acute leukaemias yet do not transform
chicken fibroblasts in culture: E26, which causes erythroblastosis and a low level of …