Single-molecule localization microscopy

M Lelek, MT Gyparaki, G Beliu, F Schueder… - Nature reviews …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Super-resolution microscopy for structural cell biology

S Liu, P Hoess, J Ries - Annual review of biophysics, 2022 - annualreviews.org
Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Deep learning enables fast and dense single-molecule localization with high accuracy

A Speiser, LR Müller, P Hoess, U Matti, CJ Obara… - Nature …, 2021 - nature.com
Single-molecule localization microscopy (SMLM) has had remarkable success in imaging
cellular structures with nanometer resolution, but standard analysis algorithms require …

A review of super-resolution single-molecule localization microscopy cluster analysis and quantification methods

IM Khater, IR Nabi, G Hamarneh - Patterns, 2020 - cell.com
Single-molecule localization microscopy (SMLM) is a relatively new imaging modality,
winning the 2014 Nobel Prize in Chemistry, and considered as one of the key super …

A phosphoinositide switch mediates exocyst recruitment to multivesicular endosomes for exosome secretion

DA Liu, K Tao, B Wu, Z Yu, M Szczepaniak… - Nature …, 2023 - nature.com
Exosomes are secreted to the extracellular milieu when multivesicular endosomes (MVEs)
dock and fuse with the plasma membrane. However, MVEs are also known to fuse with …

A survey on applications of deep learning in microscopy image analysis

Z Liu, L Jin, J Chen, Q Fang, S Ablameyko, Z Yin… - Computers in biology …, 2021 - Elsevier
Advanced microscopy enables us to acquire quantities of time-lapse images to visualize the
dynamic characteristics of tissues, cells or molecules. Microscopy images typically vary in …

Super-resolution fight club: assessment of 2D and 3D single-molecule localization microscopy software

D Sage, TA Pham, H Babcock, T Lukes, T Pengo… - Nature …, 2019 - nature.com
With the widespread uptake of two-dimensional (2D) and three-dimensional (3D) single-
molecule localization microscopy (SMLM), a large set of different data analysis packages …

Field-dependent deep learning enables high-throughput whole-cell 3D super-resolution imaging

S Fu, W Shi, T Luo, Y He, L Zhou, J Yang, Z Yang… - Nature …, 2023 - nature.com
Single-molecule localization microscopy in a typical wide-field setup has been widely used
for investigating subcellular structures with super resolution; however, field-dependent …

Single-frame deep-learning super-resolution microscopy for intracellular dynamics imaging

R Chen, X Tang, Y Zhao, Z Shen, M Zhang… - Nature …, 2023 - nature.com
Single-molecule localization microscopy (SMLM) can be used to resolve subcellular
structures and achieve a tenfold improvement in spatial resolution compared to that …

DeepSTORM3D: dense 3D localization microscopy and PSF design by deep learning

E Nehme, D Freedman, R Gordon, B Ferdman… - Nature …, 2020 - nature.com
An outstanding challenge in single-molecule localization microscopy is the accurate and
precise localization of individual point emitters in three dimensions in densely labeled …