Computer-assisted sperm analysis (CASA): Capabilities and potential developments

RP Amann, D Waberski - Theriogenology, 2014 - Elsevier
Computer-assisted sperm analysis (CASA) systems have evolved over approximately 40
years, through advances in devices to capture the image from a microscope, huge increases …

Taking aim at moving targets in computational cell migration

P Masuzzo, M Van Troys, C Ampe, L Martens - Trends in cell biology, 2016 - cell.com
Cell migration is central to the development and maintenance of multicellular organisms.
Fundamental understanding of cell migration can, for example, direct novel therapeutic …

Human sperm uses asymmetric and anisotropic flagellar controls to regulate swimming symmetry and cell steering

H Gadêlha, P Hernández-Herrera, F Montoya… - Science …, 2020 - science.org
Flagellar beating drives sperm through the female reproductive tract and is vital for
reproduction. Flagellar waves are generated by thousands of asymmetric molecular …

Rapid sperm capture: high-throughput flagellar waveform analysis

MT Gallagher, G Cupples, EH Ooi… - Human …, 2019 - academic.oup.com
STUDY QUESTION Can flagellar analyses be scaled up to provide automated tracking of
motile sperm, and does knowledge of the flagellar waveform provide new insight not …

Micro-Meta App: an interactive tool for collecting microscopy metadata based on community specifications

A Rigano, S Ehmsen, SU Öztürk, J Ryan, A Balashov… - Nature …, 2021 - nature.com
For quality, interpretation, reproducibility and sharing value, microscopy images should be
accompanied by detailed descriptions of the conditions that were used to produce them …

CASA: tracking the past and plotting the future

MT Gallagher, DJ Smith… - Reproduction, Fertility …, 2018 - CSIRO Publishing
The human semen sample carries a wealth of information of varying degrees of accessibility
ranging from the traditional visual measures of count and motility to those that need a more …

Human sperm rotate with a conserved direction during free swimming in four dimensions

G Corkidi, F Montoya, AL González-Cota… - Journal of Cell …, 2023 - journals.biologists.com
Head rotation in human spermatozoa is essential for different swimming modes and
fertilisation, as it links the molecular workings of the flagellar beat with sperm motion in three …

Imaging of the 3D dynamics of flagellar beating in human sperm

F Silva-Villalobos, JA Pimentel… - 2014 36th Annual …, 2014 - ieeexplore.ieee.org
The study of the mechanical and environmental factors that regulate a fundamental event
such as fertilization have been subject of multiple studies. Nevertheless, the microscopical …

Are there intracellular Ca2+ oscillations correlated with flagellar beating in human sperm? A three vs. two-dimensional analysis

G Corkidi, F Montoya… - MHR: Basic science …, 2017 - academic.oup.com
STUDY QUESTION Are there intracellular Ca2+ ([Ca2+] i) oscillations correlated with
flagellar beating in human sperm? SUMMARY ANSWER The results reveal statistically …

Micro-Meta App: an interactive software tool to facilitate the collection of microscopy metadata based on community-driven specifications

A Rigano, S Ehmsen, SU Ozturk, J Ryan, A Balashov… - BioRxiv, 2021 - biorxiv.org
For the information content of microscopy images to be appropriately interpreted,
reproduced, and meet FAIR (Findable Accessible Interoperable and Reusable) principles …