Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

State-of-the-art tissue-clearing methods provide subcellular-level optical access to intact
tissues from individual organs and even to some entire mammals. When combined with light …

Brains contain networks of interconnected neurons and so knowing the network architecture
is essential for understanding brain function. We therefore mapped the synaptic-resolution …

The fruit fly Drosophila melanogaster has emerged as a key model organism in
neuroscience, in large part due to the concentration of collaboratively generated molecular …

The neural circuits responsible for animal behavior remain largely unknown. We summarize
new methods and present the circuitry of a large fraction of the brain of the fruit fly …

An animal's nervous system changes as its body grows from birth to adulthood and its
behaviours mature,,,,,,–. The form and extent of circuit remodelling across the connectome is …

Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …

Drosophila melanogaster has a rich repertoire of innate and learned behaviors. Its 100,000-
neuron brain is a large but tractable target for comprehensive neural circuit mapping. Only …

Associating stimuli with positive or negative reinforcement is essential for survival, but a
complete wiring diagram of a higher-order circuit supporting associative memory has not …

Circuits in the cerebral cortex consist of thousands of neurons connected by millions of
synapses. A precise understanding of these local networks requires relating circuit activity …