Cells navigate environments, communicate and build complex patterns by initiating gene
expression in response to specific signals. Engineers seek to harness this capability to …

Highlights•Synthetic microbial communities have many potential uses in industrial
biotechnology.•Defined intercellular and spatiotemporal coordination enable precise …

Bacteria can be engineered to function as diagnostics or therapeutics in the mammalian gut
but commercial translation of technologies to accomplish this has been hindered by the …

Large genetic engineering projects require more cistrons and consequently more strong and
reliable transcriptional terminators. We have measured the strengths of a library of …

Genetic circuits require many regulatory parts in order to implement signal processing or
execute algorithms in cells. A potentially scalable approach is to use dC as9, which employs …

Optogenetic tools use colored light to rapidly control gene expression in space and time. We
designed a genetically encoded system that gives Escherichia coli the ability to distinguish …

Golden Gate cloning is a prominent DNA assembly tool in synthetic biology for the assembly
of plasmid constructs often used in combinatorial pathway optimization, with a number of …

Developing predictive models of multi‐protein genetic systems to understand and optimize
their behavior remains a combinatorial challenge, particularly when measurement …

Yarrowia lipolytica is recognized as an excellent non-conventional yeast in the field of
biomanufacturing, where it is used as a host to produce oleochemicals, terpenes, organic …

The evolutionary stability of synthetic genetic circuits is key to both the understanding and
application of genetic control elements. One useful but challenging situation is a switch …