Experimental approaches for investigating disulfide-based redox relays in cells

JD West - Chemical research in toxicology, 2022 - ACS Publications
Reversible oxidation of cysteine residues within proteins occurs naturally during normal
cellular homeostasis and can increase during oxidative stress. Cysteine oxidation often …

Characterizing interactions of endoplasmic reticulum resident proteins in situ through the YST‐PPI method

X Fan, H He, T Wang, P Xu, F Zhang, S Hu… - Biotechnology …, 2024 - Wiley Online Library
The mutual interactions of endoplasmic reticulum (ER) resident proteins in the ER maintain
its functions, prompting the protein folding, modification, and transportation. Here, a new …

The effect of the bgs13 mutation on the structure of the reporter protein beta-lactoglobulin: Influence on folding and aggregation in Pichia pastoris

B Irshad, C Lu, AE Nederstigt, P Hu, L Xue… - Protein Expression and …, 2023 - Elsevier
Pichia pastoris, a methylotrophic yeast used for recombinant protein expression, has the
capability of performing many eukaryotic post-translational modifications, growing to high …

Characterizing interactions of ER resident proteins in situ through the YST-PPI method

X Fan, H He, T Wang, P Xu, F Zhang, S Hu, Y Yun… - bioRxiv, 2024 - biorxiv.org
The mutual interactions of ER resident proteins in the ER maintain its functions, prompting
the protein folding, modification, and transportation. Here, a new method, named YST-PPI …