Evolution of Escherichia coli Expression System in Producing Antibody Recombinant Fragments
A Sandomenico, JP Sivaccumar, M Ruvo - International Journal of …, 2020 - mdpi.com
Antibodies and antibody-derived molecules are continuously developed as both therapeutic
agents and key reagents for advanced diagnostic investigations. Their application in these …
agents and key reagents for advanced diagnostic investigations. Their application in these …
Structure, dynamics and transferability of the metal-dependent polyhistidine tetramerization motif TetrHis for single-chain Fv antibodies
RD Healey, L Couillaud, F Hoh, A Mouhand… - Communications …, 2023 - nature.com
The polyhistidine (6XHis) motif is one of the most ubiquitous protein purification tags. The
6XHis motif enables the binding of tagged proteins to various metals, which can be …
6XHis motif enables the binding of tagged proteins to various metals, which can be …
Cyclization of single-chain Fv antibodies markedly suppressed their characteristic aggregation mediated by inter-chain VH-VL interactions
S Yamauchi, Y Kobashigawa, N Fukuda, M Teramoto… - Molecules, 2019 - mdpi.com
Single-chain Fv (scFv) antibodies are recombinant proteins in which the variable regions of
the heavy chain (VH) and light chain (VL) are connected by a short flexible polypeptide …
the heavy chain (VH) and light chain (VL) are connected by a short flexible polypeptide …
Preparation of single-chain Fv antibodies in the cytoplasm of Escherichia coli by simplified and systematic chaperone optimization
C Liu, Y Kobashigawa, S Yamauchi… - The journal of …, 2019 - academic.oup.com
A single-chain variable fragment (scFv) antibody is a recombinant protein in which a peptide
linker connects the variable regions of the heavy chain and light chain. Due to its smaller …
linker connects the variable regions of the heavy chain and light chain. Due to its smaller …
Molecular dynamics-based design and biophysical evaluation of thermostable single-chain Fv antibody mutants derived from pharmaceutical antibodies
K Okazaki, Y Kobashigawa, H Morita, S Yamauchi… - ACS …, 2023 - ACS Publications
Antibody drugs are denatured under physical stress, eg, friction, heat, and freezing, which
triggers formation of aggregates and resultant allergic reactions. Design of a stable antibody …
triggers formation of aggregates and resultant allergic reactions. Design of a stable antibody …
Evaluation of the cyclic single-chain Fv antibody derived from nivolumab by biophysical analyses and in vitro cell-based bioassay
S Kamesawa, M Ogawa, Y Funakoshi… - The Journal of …, 2024 - academic.oup.com
Single-chain Fv (scFv) is a recombinant small antibody in which a polypeptide linker
connects the variable regions of the light chain (VL) and the heavy chain (VH). The practical …
connects the variable regions of the light chain (VL) and the heavy chain (VH). The practical …
Convenient method of producing cyclic single-chain Fv antibodies by split-intein-mediated protein ligation and chaperone co-expression
C Liu, Y Kobashigawa, S Yamauchi… - The journal of …, 2020 - academic.oup.com
Single-chain Fv (scFv) is a recombinant antibody in which the variable regions of the heavy
chain (VH) and light chain (VL) are connected by a short flexible polypeptide linker …
chain (VH) and light chain (VL) are connected by a short flexible polypeptide linker …
Molecular recognition of a single-chain Fv antibody specific for GA-pyridine, an advanced glycation end-product (AGE), elucidated using biophysical techniques and …
Y Kobashigawa, T Ohara, K Morita… - The journal of …, 2021 - academic.oup.com
Advanced glycation end-products (AGEs) are a heterogeneous group of compounds formed
by non-enzymatic reaction between reducing-sugar and Arg/Lys in proteins and are …
by non-enzymatic reaction between reducing-sugar and Arg/Lys in proteins and are …
[PDF][PDF] Structure and Dynamics of Tetrhis, a Novel Metal-dependent Polyhistidine Tetramerization Motif
R Healey, L Couillaud, F Hoh, A Mouhand, S Granier… - 2023 - hal.umontpellier.fr
The polyhistidine (6XHis) tag is one of the most ubiquitous fusion tags used for the puri
cation of recombinant proteins. The 6XHis motif enables the tagged protein to bind metal …
cation of recombinant proteins. The 6XHis motif enables the tagged protein to bind metal …
Overview of Bioimaging with HS-AFM
T Ando - High-Speed Atomic Force Microscopy in Biology …, 2022 - Springer
This chapter gives an overview of bioimaging studies with HS-AFM performed in recent
years, efficiently illustrating how this new field has evolved and what biomolecular …
years, efficiently illustrating how this new field has evolved and what biomolecular …