The paradigm of regenerative medicine has recently shifted from in vitro to in situ tissue
engineering: implanting a cell-free, biodegradable, off-the-shelf available scaffold and …

Stem cells have an important role in regenerative therapies, developmental biology studies
and drug screening. Basic and translational research in stem cell technology needs more …

Tissue-engineered blood vessels (TEVs) are typically produced using the pulsatile, uniaxial
circumferential stretch to mechanically condition and strengthen the arterial grafts. Despite …

Three-dimensional (3D) cell culture is an important tool that facilitates biological discoveries
by bridging the divide between standard two-dimensional cell culture and the complex, high …

Three-dimensional stroma engineered models would enable fundamental and applicative
studies of human tissues interaction and remodeling in both physiological and pathological …

Visualizing the three-dimensional morphology and spatial patterning of cells embedded
deep within dense connective tissues of the musculoskeletal system has been possible only …

Three-dimensional (3-D) in vitro platforms have been shown to closely recapitulate human
physiology when compared with conventional two-dimensional (2-D) in vitro or in vivo …

Confocal and multiphoton microscopy are effective techniques to obtain high-contrast
images of 2-D sections within bulk tissue. However, scattering limits their application to …

In the field of tissue engineering, there is a need for methods that allow assessing the
performance of tissue-engineered constructs noninvasively in vitro and in vivo. To date …

This study investigates osteoblastic cell spheroid cultivation methods, exploring flat-bottom,
U-bottom, and rotary flask techniques with and without amorphous calcium phosphate (ACP) …