Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …
Fluorescence lifetime imaging (FLIM): Basic concepts and some recent developments
K Suhling, LM Hirvonen, JA Levitt, PH Chung… - Medical photonics, 2015 - Elsevier
Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …
environment and interaction of fluorescent probes. It can report on photophysical events that …
FXR/TGR5 dual agonist prevents progression of nephropathy in diabetes and obesity
Bile acids are ligands for the nuclear hormone receptor farnesoid X receptor (FXR) and the
G protein–coupled receptor TGR5. We have shown that FXR and TGR5 have renoprotective …
G protein–coupled receptor TGR5. We have shown that FXR and TGR5 have renoprotective …
Intraoperative margin assessment in oral and oropharyngeal cancer using label-free fluorescence lifetime imaging and machine learning
Objective: To demonstrate the diagnostic ability of label-free, point-scanning, fiber-based
Fluorescence Lifetime Imaging (FLIm) as a means of intraoperative guidance during oral …
Fluorescence Lifetime Imaging (FLIm) as a means of intraoperative guidance during oral …
Label-free super-resolution imaging techniques
RE Leighton, AM Alperstein… - Annual Review of …, 2022 - annualreviews.org
Biological and material samples contain nanoscale heterogeneities that are unresolvable
with conventional microscopy techniques. Super-resolution fluorescence methods can break …
with conventional microscopy techniques. Super-resolution fluorescence methods can break …
Fluorescence, phosphorescence, and chemiluminescence
Fluorescence, Phosphorescence, and Chemiluminescence cover a wide array of topics.
One can simply examine the number of topics that arise from photon induced excitation and …
One can simply examine the number of topics that arise from photon induced excitation and …
Segmented cell analyses to measure redox states of autofluorescent NAD (P) H, FAD & Trp in cancer cells by FLIM
Multiphoton FLIM microscopy offers many opportunities to investigate processes in live cells,
tissue and animal model systems. For redox measurements, FLIM data is mostly published …
tissue and animal model systems. For redox measurements, FLIM data is mostly published …
Mesoscopic fluorescence lifetime imaging: Fundamental principles, clinical applications and future directions
Fluorescence lifetime imaging (FLIm) is an optical spectroscopic imaging technique capable
of real‐time assessments of tissue properties in clinical settings. Label‐free FLIm is sensitive …
of real‐time assessments of tissue properties in clinical settings. Label‐free FLIm is sensitive …
Simultaneous NAD (P) H and FAD fluorescence lifetime microscopy of long UVA–induced metabolic stress in reconstructed human skin
Solar ultraviolet longwave UVA1 exposure of human skin has short-term consequences at
cellular and molecular level, leading at long-term to photoaging. Following exposure …
cellular and molecular level, leading at long-term to photoaging. Following exposure …
Real‐time augmented reality for delineation of surgical margins during neurosurgery using autofluorescence lifetime contrast
A Alfonso‐Garcia, J Bec… - Journal of …, 2020 - Wiley Online Library
Current clinical brain imaging techniques used for surgical planning of tumor resection lack
intraoperative and real‐time feedback; hence surgeons ultimately rely on subjective …
intraoperative and real‐time feedback; hence surgeons ultimately rely on subjective …