Since its inception, the CRISPR/Cas9 technology has been widely utilized for the targeted
insertion of donor DNAs into mammalian genomes. A shortcoming with the earlier knock-in …

In the present day, it is possible to incorporate targeted mutations or replace a gene using
genome editing techniques such as customisable CRISPR/Cas9 system. Although induction …

The ease and efficacy of CRISPR/Cas9 germline gene editing in animal models paved the
way to human germline gene editing (HGGE), by which permanent changes can be …

The specificity and efficiency of CRISPR/Cas9 gene-editing systems are determined by
several factors, including the mode of delivery, when applied to mammalian embryos. Given …

The introduction of genome editing reagents into mammalian zygotes has traditionally been
accomplished by cytoplasmic or pronuclear microinjection. This time-consuming procedure …

Recent advances in CRISPR-Cas genome editing technology have been instrumental in
improving the efficiency to produce genetically modified animal models. In this study we …

Simple Summary Liposome-mediated gene transfer has become an alternative method for
establishing a gene targeting framework, and the production of mutant animals may be …

Gene-engineered animals created using gene-targeting technology have long been
recognized as beneficial, valid, and valuable tools for exploring the function of a gene of …

Genetic improvement of livestock is critical for animal production and wellbeing. Traditional
breeding approaches however are slow and often result in unwanted genetic linkage drag …