The use of enzymes in industrial processes requires the improvement of their features in
many instances. Enzyme immobilization, a requirement to facilitate the recovery and reuse …

In this review, we detail the efforts performed to couple the purification and the
immobilization of industrial enzymes in a single step. The use of antibodies, the …

A heterofunctional support for enzyme immobilization may be defined as that which
possesses several distinct functionalities on its surface able to interact with a protein. We will …

The role of ionized groups in biological systems is determined by their affinity for water
[Biophys. J. 72 (1997) 65–76]. The tightly bound water associated with biologically important …

In this article, an overview of hydrophobic interaction chromatography (HIC) of proteins is
given. After a brief description of protein hydrophobicity and hydrophobic interactions, we …

Sepabeads‐EP (a new epoxy support) has been utilized to immobilize‐stabilize the enzyme
penicillin G acylase (PGA) via multipoint covalent attachment. These supports are very …

Multifunctional supports containing epoxy groups are here proposed as a second
generation of activated supports for covalent immobilization of enzymes following the epoxy …

The properties of a new commercially available amino-epoxy support (amino-epoxy-
Sepabeads) for immobilizing enzymes have been compared to those of conventional epoxy …

Simple salts are known to influence the cloud point temperature of the aqueous solution of
thermoresponsive compounds, such as poly-(N-isopropylacrylamide), PNIPAM. The effect of …

The effect of salt on the retention behavior of proteins in electrostatic and hydrophobic
interaction chromatography is described by a three-parameter equation, log k′= AB log m …