Strategies for culture of 'unculturable'bacteria

SR Vartoukian, RM Palmer… - FEMS microbiology …, 2010 - academic.oup.com
Molecular ecology methods are now well established for the culture-independent
characterization of complex bacterial communities associated with various environmental …

Cultivation of unculturable soil bacteria

VHT Pham, J Kim - Trends in biotechnology, 2012 - cell.com
Despite the abundance of bacterial species in soil, more than 99% of these species cannot
be cultured by traditional techniques. In addition, the less than 1% of bacteria that can be …

How many antibiotics are produced by the genus Streptomyces?

MG Watve, R Tickoo, MM Jog, BD Bhole - Archives of microbiology, 2001 - Springer
Streptomyces is the largest antibiotic-producing genus in the microbial world discovered so
far. The number of antimicrobial compounds reported from the species of this genus per year …

Rare actinomycetes: a potential storehouse for novel antibiotics

K Tiwari, RK Gupta - Critical reviews in biotechnology, 2012 - Taylor & Francis
New antimicrobial agents are desperately needed to combat the increasing number of
antibiotic resistant strains of pathogenic microorganisms. Natural products remain the most …

Cultivating the uncultured: limits, advances and future challenges

K Alain, J Querellou - Extremophiles, 2009 - Springer
Since the invention of the Petri dish, there have been continuous efforts to improve efficiency
in microbial cultivation. These efforts were devoted to the attainment for diverse growth …

Incubation of environmental samples in a diffusion chamber increases the diversity of recovered isolates

A Bollmann, K Lewis, SS Epstein - Applied and environmental …, 2007 - Am Soc Microbiol
The majority of microorganisms from natural environments cannot be grown in the
laboratory. The diffusion-chamber-based approach is an alternative method that allows …

From axenic to mixed cultures: technological advances accelerating a paradigm shift in microbiology

C Nai, V Meyer - Trends in microbiology, 2018 - cell.com
Since the onset of microbiology in the late 19th century, scientists have been growing
microorganisms almost exclusively as pure cultures, resulting in a limited and biased view of …

Microcolony cultivation on a soil substrate membrane system selects for previously uncultured soil bacteria

BC Ferrari, SJ Binnerup, M Gillings - Applied and environmental …, 2005 - Am Soc Microbiol
Traditional microbiological methods of cultivation recover less than 1% of the total bacterial
species, and the culturable portion of bacteria is not representative of the total phylogenetic …

Using “omics” and integrated multi-omics approaches to guide probiotic selection to mitigate chytridiomycosis and other emerging infectious diseases

EA Rebollar, RE Antwis, MH Becker… - Frontiers in …, 2016 - frontiersin.org
Emerging infectious diseases in wildlife are responsible for massive population declines. In
amphibians, chytridiomycosis caused by Batrachochytrium dendrobatidis, Bd, has severely …

Culture enriched molecular profiling of the cystic fibrosis airway microbiome

CD Sibley, ME Grinwis, TR Field, CS Eshaghurshan… - PloS one, 2011 - journals.plos.org
The microbiome of the respiratory tract, including the nasopharyngeal and oropharyngeal
microbiota, is a dynamic community of microorganisms that is highly diverse. The cystic …