Membrane mimetic systems in CryoEM: keeping membrane proteins in their native environment
Highlights•Single-particle cryoEM facilitates structural determination of membrane proteins
without the need of crystallization.•Advances in membrane protein sample preparation are …
without the need of crystallization.•Advances in membrane protein sample preparation are …
Recent advances in nanodisc technology for membrane protein studies (2012–2017)
Historically, the main barrier to membrane protein investigations has been the tendency of
membrane proteins to aggregate (due to their hydrophobic nature), in aqueous solution as …
membrane proteins to aggregate (due to their hydrophobic nature), in aqueous solution as …
The 3.5-Å CryoEM structure of nanodisc-reconstituted yeast vacuolar ATPase Vo proton channel
The molecular mechanism of transmembrane proton translocation in rotary motor ATPases
is not fully understood. Here, we report the 3.5-Å resolution cryoEM structure of the lipid …
is not fully understood. Here, we report the 3.5-Å resolution cryoEM structure of the lipid …
Oxidative stress protein Oxr1 promotes V‐ATPase holoenzyme disassembly in catalytic activity‐independent manner
MM Khan, S Lee, S Couoh‐Cardel, RA Oot… - The EMBO …, 2022 - embopress.org
Abstract The vacuolar ATPase (V‐ATPase) is a rotary motor proton pump that is regulated by
an assembly equilibrium between active holoenzyme and autoinhibited V1‐ATPase and Vo …
an assembly equilibrium between active holoenzyme and autoinhibited V1‐ATPase and Vo …
Structural comparison of the vacuolar and Golgi V-ATPases from Saccharomyces cerevisiae
T Vasanthakumar, SA Bueler, DI Wu… - Proceedings of the …, 2019 - National Acad Sciences
Proton-translocating vacuolar-type ATPases (V-ATPases) are necessary for numerous
processes in eukaryotic cells, including receptor-mediated endocytosis, protein maturation …
processes in eukaryotic cells, including receptor-mediated endocytosis, protein maturation …
Detection and quantification of the vacuolar H+ATPase using the Legionella effector protein SidK
Acidification of secretory and endocytic organelles is required for proper receptor recycling,
membrane traffic, protein degradation, and solute transport. Proton-pumping vacuolar H+ …
membrane traffic, protein degradation, and solute transport. Proton-pumping vacuolar H+ …
Real-Time Measurement of a Weak Interaction of a Transcription Factor Motif with a Protein Hub at Single-Molecule Precision
Transcription factors often interact with other protein cofactors, regulating gene expression.
Direct detection of these brief events using existing technologies remains challenging due to …
Direct detection of these brief events using existing technologies remains challenging due to …
Molecular basis for the binding and modulation of V-ATPase by a bacterial effector protein
Intracellular pathogenic bacteria evade the immune response by replicating within host
cells. Legionella pneumophila, the causative agent of Legionnaires' Disease, makes use of …
cells. Legionella pneumophila, the causative agent of Legionnaires' Disease, makes use of …
Evaluation of nanopore sensor design using electrical and optical analyses
Nanopores are currently utilized as powerful tools for single-molecule protein sensing. The
reporting signal typically requires protein analytes to enter the nanopore interior, yet a class …
reporting signal typically requires protein analytes to enter the nanopore interior, yet a class …
Structure of V-ATPase from citrus fruit
We used the Legionella pneumophila effector SidK to affinity purify the endogenous
vacuolar-type ATPases (V-ATPases) from lemon fruit. The preparation was sufficient for …
vacuolar-type ATPases (V-ATPases) from lemon fruit. The preparation was sufficient for …