The cyclin-dependent protein kinase (CDK) encoded by CDC28 is the master regulator of
cell division in the budding yeast Saccharomyces cerevisiae. By mechanisms that, for the …

The ability of cells to make exact replicas of themselves is central to the life and
development of complex organisms. Initial insights into the question of how cells divide …

The mitotic checkpoint prevents cells with unaligned chromosomes from prematurely exiting
mitosis by inhibiting the anaphase-promoting complex/cyclosome (APC/C) from targeting …

The mitotic checkpoint blocks the activation of the anaphase-promoting complex (APC) until
all sister chromatids have achieved bipolar attachment to the spindle. A checkpoint complex …

Prior to sister-chromatid separation, the spindle checkpoint inhibits cell-cycle progression in
response to a signal generated by mitotic spindle damage or by chromosomes that have not …

Polo kinases play critical roles for proper M-phase progression. They are characterized by
the presence of two regions of homology in the C-terminal non-catalytic domain, termed polo …

Aneuploidy is a characteristic of the majority of human cancers, and recent work has
suggested that mitotic checkpoint defects play a role in its development. To further explore …

The spindle checkpoint delays the onset of anaphase until all pairs of sister chromatids are
attached to the mitotic spindle. The checkpoint could monitor the attachment of microtubules …

In yeast, anaphase entry depends on Pds1 proteolysis, while chromosome re-duplication in
the subsequent S-phase involves degradation of mitotic cyclins such as Clb2. Sequential …

In yeast, the Mad2 protein is required for the M phase arrest induced by microtubule
inhibitors, but the protein is not essential under normal culture conditions. We tested whether …