[PDF][PDF] Methods of plant transformation-a review

G Keshavareddy, ARV Kumar… - Int. J. Curr. Microbiol. Appl …, 2018 - academia.edu
Plant genetic transformation permits direct introduction of agronomically useful genes into
important crops and offers a significant tool in breeding programs by producing novel and …

Advances in Agrobacterium tumefaciens-mediated genetic transformation of graminaceous crops

RK Singh, M Prasad - Protoplasma, 2016 - Springer
Steady increase in global population poses several challenges to plant science research,
including demand for increased crop productivity, grain yield, nutritional quality and …

A protocol for Agrobacterium-mediated transformation in rice

A Nishimura, I Aichi, M Matsuoka - Nature protocols, 2006 - nature.com
Agrobacterium-mediated transformation of rice is an important method that has been widely
adopted by many laboratories. However, because current approaches rely on culture …

A fast and genotype-independent in planta Agrobacterium-mediated transformation method for soybean

H Zhong, C Li, W Yu, H Zhou, T Lieber, X Su… - Plant …, 2024 - cell.com
Efficient genotype-independent transformation and genome editing are highly desirable for
plant biotechnology research and product development efforts. We have developed a novel …

Development of simple and efficient in planta transformation method for wheat (Triticum aestivum L.) using Agrobacterium tumefaciens

P Supartana, T Shimizu, M Nogawa, H Shioiri… - Journal of bioscience …, 2006 - Elsevier
Wheat (Triticum aestivum L. var. Shiranekomugi) seeds were soaked in water at 22° C for 1
d. Thereafter, the embryo of the soaked seeds was inoculated with Agrobacterium …

Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice

J Lin, B Zhou, Y Yang, J Mei, X Zhao, X Guo, X Huang… - Plant Cell Reports, 2009 - Springer
Traditional transformation methods are complex and time consuming. It is generally difficult
to transform indica rice varieties using traditional transformation methods due to their poor …

Agrobacterium-mediated plant transformation: Factors, applications and recent advances

A Ziemienowicz - Biocatalysis and Agricultural Biotechnology, 2014 - Elsevier
Agrobacterium has been widely used as a vector to create transgenic plants. Agrobacterium-
mediated gene transfer is governed by various factors of bacterial, host and environmental …

Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane

S Mayavan, K Subramanyam, M Arun, M Rajesh… - Plant cell reports, 2013 - Springer
Key message An efficient, reproducible and genotype-independent in planta transformation
has been standardized for sugarcane using seed as explant. Abstract Transgenic sugarcane …

In planta particle bombardment (iPB): A new method for plant transformation and genome editing

R Imai, H Hamada, Y Liu, Q Linghu, Y Kumagai… - Plant …, 2020 - jstage.jst.go.jp
Transformation is a key step in modern breeding technology that involves genome editing.
The requirement for in vitro tissue culture and regeneration hampers application of this …

Piercing and incubation method of in planta transformation producing stable transgenic plants by overexpressing DREB1A gene in tomato (Solanum lycopersicum …

SH Shah, S Ali, SA Jan, GM Ali - Plant Cell, Tissue and Organ Culture …, 2015 - Springer
Cold is a major constraint for tomato growth and productivity; as it is a cold sensitive crop.
DREB1A plays a key role in generating cold tolerance in tomato by regulating the response …