The applications of Förster resonance energy transfer (FRET) grow with each year.
However, different FRET techniques are not applied consistently, nor are results uniformly …

Förster or fluorescence resonance energy transfer (FRET) technology and genetically
encoded FRET biosensors provide a powerful tool for visualizing signaling molecules in live …

Phosphorescence, characterized by luminescent lifetimes significantly longer than that of
biological autofluorescence under ambient environment, is of great value for biomedical …

The success of fluorescence experiments requires attention to experimental details and an
understanding of the instrumentation. There are also many potential artifacts that can distort …

Förster (or Fluorescence) Resonance Energy Transfer (FRET) is unique in generating
fluorescence signals sensitive to molecular conformation, association, and separation in the …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

Time-correlated single photon counting (TCSPC) is based on the detection of single photons
of a periodic light signal, measurement of the detection time of the photons, and the build-up …

Fluorescence polarization was first applied in biochemistry almost 6 decades ago, when
Gregorio Weber described his studies on bovine serum albumin and ovalbumin conjugated …

Dynamic protein interactions play a significant part in many cellular processes. A technique
that shows considerable promise in elucidating such interactions is Förster resonance …

Cholesterol and sphingolipid-enriched" rafts" have long been proposed as platforms for the
sorting of specific membrane components including glycosyl-phosphatidylinositol-anchored …