The role of proline in the adaptation of eukaryotic microalgae to environmental stress: An underestimated tool for the optimization of algal growth

S Barera, G Forlani - Journal of Applied Phycology, 2023 - Springer
Microalgae are considered the most promising source of renewable fuels, high-value bio-
products and nutraceuticals. Potentially, microalgae can satisfy many global demands, but in …

Harnessing the algal chloroplast for heterologous protein production

EA Cutolo, G Mandalà, L Dall'Osto, R Bassi - Microorganisms, 2022 - mdpi.com
Photosynthetic microbes are gaining increasing attention as heterologous hosts for the light-
driven, low-cost production of high-value recombinant proteins. Recent advances in the …

[HTML][HTML] Combinatorial engineering enables photoautotrophic growth in high cell density phosphite-buffered media to support engineered chlamydomonas reinhardtii …

MN Abdallah, GB Wellman, S Overmans… - Frontiers in …, 2022 - frontiersin.org
Chlamydomonas reinhardtii has emerged as a powerful green cell factory for metabolic
engineering of sustainable products created from the photosynthetic lifestyle of this …

Assessing the effect of a series of mutations on the dynamic behavior of phosphite dehydrogenase using molecular docking, molecular dynamics and quantum …

S Baammi, R Daoud, A El Allali - Journal of Biomolecular Structure …, 2023 - Taylor & Francis
Discovered in Pseudomonas stutzeri, phosphite dehydrogenase (PTDH) is an enzyme that
catalyzes the oxidation of phosphite to phosphate while simultaneously reducing NAD+ to …

Algae biomass as a potential source of liquid fuels

M Dębowski, M Zieliński, I Świca, J Kazimierowicz - Phycology, 2021 - mdpi.com
Algae biomass is perceived as a prospective source of many types of biofuels, including
biogas and biomethane produced in the anaerobic digestion process, ethanol from alcoholic …

The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics

EA Cutolo, R Caferri, R Campitiello, M Cutolo - Marine Drugs, 2023 - mdpi.com
Rheumatoid arthritis (RA) is an invalidating chronic autoimmune disorder characterized by
joint inflammation and progressive bone damage. Dietary intervention is an important …

Heterologous expression of formate dehydrogenase enables photoformatotrophy in the emerging model microalga, Picochlorum renovo

LR Dahlin, AW Meyers, SW Stefani… - … in Bioengineering and …, 2023 - frontiersin.org
Rising global greenhouse gas emissions and the impacts of resultant climate change
necessitate development and deployment of carbon capture and conversion technologies …

Heterologous expression of phosphite dehydrogenase in the chloroplast or nucleus enables phosphite utilization and genetic selection in Picochlorum spp.

LR Dahlin, MT Guarnieri - Algal Research, 2022 - Elsevier
Microalgae present a path to ameliorate problems associated with climate change via
capture and reduction of CO 2 to sustainable fuels and chemicals. Picochlorum is a genus of …

ptxD/Phi as alternative selectable marker system for genetic transformation for bio-safety concerns: a review

R Dormatey, C Sun, K Ali, S Fiaz, D Xu… - PeerJ, 2021 - peerj.com
Antibiotic and herbicide resistance genes are the most common marker genes for plant
transformation to improve crop yield and food quality. However, there is public concern …

A chimeric hydrolase-PTXD transgene enables chloroplast-based heterologous protein expression and non-sterile cultivation of Chlamydomonas reinhardtii

E Cutolo, M Tosoni, S Barera, L Herrera-Estrella… - Algal Research, 2021 - Elsevier
Photosynthetic microalgae hold great potential as light-driven heterologous protein
expression hosts. In particular, the algal chloroplast is an ideal sub-cellular site for the …