ThT 101: a primer on the use of thioflavin T to investigate amyloid formation

K Gade Malmos, LM Blancas-Mejia, B Weber… - Amyloid, 2017 - Taylor & Francis
Thioflavin T (ThT) has been widely used to investigate amyloid formation since 1989. While
concerns have recently been raised about its use as a probe specific for amyloid, ThT still …

Electronic circular dichroism spectroscopy of proteins

DM Rogers, SB Jasim, NT Dyer, F Auvray, M Réfrégiers… - Chem, 2019 - cell.com
Electronic circular dichroism (CD) spectroscopy is an important tool for the elucidation of
biomolecular structure. This review describes the latest progress and developments in …

A peptide-based nanofibrous hydrogel as a promising DNA nanovector for optimizing the efficacy of HIV vaccine

Y Tian, H Wang, Y Liu, L Mao, W Chen, Z Zhu, W Liu… - Nano …, 2014 - ACS Publications
This report shows that a nanovector composed of peptide-based nanofibrous hydrogel can
condense DNA to result in strong immune responses against HIV. This nanovector can …

Protein characterisation by synchrotron radiation circular dichroism spectroscopy

BA Wallace - Quarterly reviews of biophysics, 2009 - cambridge.org
Circular dichroism (CD) spectroscopy is a well-established technique for the study of
proteins. Synchrotron radiation circular dichroism (SRCD) spectroscopy extends the utility of …

Structural stability of electrosprayed proteins: temperature and hydration effects

EG Marklund, DSD Larsson, D van der Spoel… - Physical Chemistry …, 2009 - pubs.rsc.org
Electrospray ionization is a gentle method for sample delivery, routinely used in gas-phase
studies of proteins. It is crucial for structural investigations that the protein structure is …

An in vitro investigation of the inhibitory mechanism of β-galactosidase by cinnamaldehyde alone and in combination with carvacrol and thymol

LH Wang, MS Wang, XA Zeng, DM Gong… - Biochimica et Biophysica …, 2017 - Elsevier
Background Some antibacterial agents exert their antimicrobial action by targeting the
cytoplasmic macromolecules, such as proteins or nucleic acids, to disturb the properties of …

Structural and functional characterization of human peripheral nervous system myelin protein P2

V Majava, E Polverini, A Mazzini, R Nanekar, W Knoll… - PLoS …, 2010 - journals.plos.org
The myelin sheath is a tightly packed multilayered membrane structure insulating selected
axons in the central and the peripheral nervous systems. Myelin is a biochemically unique …

Synchrotron radiation circular dichroism (SRCD) spectroscopy: an enhanced method for examining protein conformations and protein interactions

BA Wallace, RW Janes - Biochemical Society Transactions, 2010 - portlandpress.com
CD (circular dichroism) spectroscopy is a well-established technique in structural biology.
SRCD (synchrotron radiation circular dichroism) spectroscopy extends the utility and …

Emerging methods for structural analysis of protein aggregation

E Khan, SK Mishra, A Kumar - Protein and peptide letters, 2017 - ingentaconnect.com
Protein misfolding and aggregation is a key attribute of different neurodegenerative
diseases. Misfolded and aggregated proteins are intrinsically disordered and rule out …

[PDF][PDF] UV-CD12: synchrotron radiation circular dichroism beamline at ANKA

J Bürck, S Roth, D Windisch, P Wadhwani… - Journal of …, 2015 - journals.iucr.org
Synchrotron radiation circular dichroism (SRCD) is a rapidly growing technique for structure
analysis of proteins and other chiral biomaterials. UV-CD12 is a high-flux SRCD beamline …