Antibody‐incorporated nanomedicines for cancer therapy

SY Wu, FG Wu, X Chen - Advanced Materials, 2022 - Wiley Online Library
Antibody‐based cancer therapy, one of the most significant therapeutic strategies, has
achieved considerable success and progress over the past decades. Nevertheless …

Power to the protein: enhancing and combining activities using the Spy toolbox

AH Keeble, M Howarth - Chemical Science, 2020 - pubs.rsc.org
Proteins span an extraordinary range of shapes, sizes and functionalities. Therefore generic
approaches are needed to overcome this diversity and stream-line protein analysis or …

Site-specific biotinylation of purified proteins using BirA

M Fairhead, M Howarth - Site-Specific Protein Labeling: Methods and …, 2015 - Springer
The binding between biotin and streptavidin or avidin is one of the strongest known non-
covalent biological interactions. The (strept) avidin-biotin interaction has been widely used …

Bio-SCAN: A CRISPR/dCas9-based lateral flow assay for rapid, specific, and sensitive detection of SARS-CoV-2

Z Ali, E Sánchez, M Tehseen, A Mahas… - ACS Synthetic …, 2021 - ACS Publications
Simple, rapid, specific, and sensitive point-of-care detection methods are needed to contain
the spread of SARS-CoV-2. CRISPR/Cas9-based lateral flow assays are emerging as a …

The principles and applications of avidin-based nanoparticles in drug delivery and diagnosis

A Jain, K Cheng - Journal of controlled release, 2017 - Elsevier
Avidin-biotin interaction is one of the strongest non-covalent interactions in the nature.
Avidin and its analogues have therefore been extensively utilized as probes and affinity …

Optical tweezers—from calibration to applications: a tutorial

J Gieseler, JR Gomez-Solano, A Magazzù… - Advances in Optics …, 2021 - opg.optica.org
Since their invention in 1986 by Arthur Ashkin and colleagues, optical tweezers have
become an essential tool in several fields of physics, spectroscopy, biology …

Aptamers from random sequence space: Accomplishments, gaps and future considerations

S Qian, D Chang, S He, Y Li - Analytica Chimica Acta, 2022 - Elsevier
Aptamers are molecular recognition elements made of nucleic acids. Diverse synthetic
aptamers have been discovered in a large number of SELEX experiments since 1990. This …

Graphene field-effect transistor biosensor for detection of biotin with ultrahigh sensitivity and specificity

S Wang, MZ Hossain, K Shinozuka, N Shimizu… - Biosensors and …, 2020 - Elsevier
Because avidin and biotin molecules exhibit the most specific and strongest non-covalent
interaction, avidin-biotin technology is widely used in ELISA (enzyme-linked immunosorbent …

New routes and opportunities for modular construction of particulate vaccines: stick, click, and glue

KD Brune, M Howarth - Frontiers in immunology, 2018 - frontiersin.org
Vaccines based on virus-like particles (VLPs) can induce potent B cell responses. Some non-
chimeric VLP-based vaccines are highly successful licensed products (eg, hepatitis B …

[HTML][HTML] Multivalent protein-drug conjugates–An emerging strategy for the upgraded precision and efficiency of drug delivery to cancer cells

N Porębska, K Ciura, A Chorążewska… - Biotechnology …, 2023 - Elsevier
With almost 20 million new cases per year, cancer constitutes one of the most important
challenges for public health systems. Unlike traditional chemotherapy, targeted anti-cancer …