The real-time polymerase chain reaction
M Kubista, JM Andrade, M Bengtsson… - Molecular aspects of …, 2006 - Elsevier
The scientific, medical, and diagnostic communities have been presented the most powerful
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …
tool for quantitative nucleic acids analysis: real-time PCR [Bustin, SA, 2004. A–Z of …
Quantitative real-time RT-PCR–a perspective
The real-time reverse transcription polymerase chain reaction (RT-PCR) uses fluorescent
reporter molecules to monitor the production of amplification products during each cycle of …
reporter molecules to monitor the production of amplification products during each cycle of …
The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Background: Currently, a lack of consensus exists on how best to perform and interpret
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
quantitative real-time PCR (qPCR) experiments. The problem is exacerbated by a lack of …
Group‐specific primer and probe sets to detect methanogenic communities using quantitative real‐time polymerase chain reaction
Y Yu, C Lee, J Kim, S Hwang - Biotechnology and …, 2005 - Wiley Online Library
Real‐time polymerase chain reaction (PCR) is a highly sensitive method that can be used
for the detection and quantification of microbial populations without cultivating them in …
for the detection and quantification of microbial populations without cultivating them in …
[PDF][PDF] Quantification strategies in real-time PCR
MW Pfaffl - AZ of quantitative PCR, 2004 - Citeseer
This chapter analyzes the quantification strategies in real-time RT-PCR and all
corresponding markers of a successful real-time RT-PCR. The following aspects are …
corresponding markers of a successful real-time RT-PCR. The following aspects are …
High-resolution genotyping by amplicon melting analysis using LCGreen
CT Wittwer, GH Reed, CN Gundry… - Clinical …, 2003 - academic.oup.com
Background: High-resolution amplicon melting analysis was recently introduced as a closed-
tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003; 49: 396 …
tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003; 49: 396 …
Point-of-care diagnostics for global health
P Yager, GJ Domingo, J Gerdes - Annu. Rev. Biomed. Eng., 2008 - annualreviews.org
Biomedical engineers have traditionally developed technologies in response to the needs of
the developed world's medical community. As a result, the diagnostic systems on which they …
the developed world's medical community. As a result, the diagnostic systems on which they …
[HTML][HTML] Real-time PCR in the microbiology laboratory
IM Mackay - Clinical microbiology and infection, 2004 - Elsevier
Use of PCR in the field of molecular diagnostics has increased to the point where it is now
accepted as the standard method for detecting nucleic acids from a number of sample and …
accepted as the standard method for detecting nucleic acids from a number of sample and …
FilmArray, an automated nested multiplex PCR system for multi-pathogen detection: development and application to respiratory tract infection
The ideal clinical diagnostic system should deliver rapid, sensitive, specific and reproducible
results while minimizing the requirements for specialized laboratory facilities and skilled …
results while minimizing the requirements for specialized laboratory facilities and skilled …
High-resolution DNA melting analysis for simple and efficient molecular diagnostics
GH Reed, JO Kent, CT Wittwer - Pharmacogenomics, 2007 - Taylor & Francis
High-resolution melting of DNA is a simple solution for genotyping, mutation scanning and
sequence matching. The melting profile of a PCR product depends on its GC content, length …
sequence matching. The melting profile of a PCR product depends on its GC content, length …