Cryogenic electron microscopy (cryo-EM) data represent density maps of macromolecular
systems at atomic or near-atomic resolution. However, building and refining 3D atomic …

Coot is a tool widely used for model building, refinement, and validation of macromolecular
structures. It has been extensively used for crystallography and, more recently …

DNA and RNA play fundamental roles in various cellular processes, where their three-
dimensional structures provide information critical to understanding the molecular …

The recent rapid development of single-particle electron cryo-microscopy (cryo-EM) now
allows structures to be solved by this method at resolutions close to 3 Å. Here, a number of …

Mitochondria have specialized ribosomes that have diverged from their bacterial and
cytoplasmic counterparts. We have solved the structure of the yeast mitoribosomal large …

Mechanistic understanding of pre-mRNA splicing requires detailed structural information on
various states of the spliceosome. Here we report the cryo electron microscopy (cryo-EM) …

Pre–messenger RNA (pre-mRNA) splicing is carried out by the spliceosome, which
undergoes an intricate assembly and activation process. Here, we report an atomic structure …

Advances in our understanding of macromolecular structure come from experimental
methods, such as X-ray crystallography, and also computational analysis of the growing …

The molecular mechanisms of exon definition and back-splicing are fundamental
unanswered questions in pre-mRNA splicing. Here we report cryo-electron microscopy …

Cryo-EM structure determination of protein-free RNAs has remained difficult with most
attempts yielding low to moderate resolution and lacking nucleotide-level detail. These …