When a molecule absorbs a photon of appropriate energy, a chain of photophysical events
ensues, such as internal conversion or vibrational relaxation (loss of energy in the absence …

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging tool offering
molecular specific insights into samples through the measurement of fluorescence decay …

Fluorescence lifetime imaging microscopy (FLIM) is now routinely used for dynamic
measurements of signaling events inside living cells, including detection of protein-protein …

Live cells function within narrow limits of physiological temperature (T) and O2 and
metabolite concentrations. We have designed a cell-permeable T-sensitive fluorescence …

Time-resolved three-dimensional diagnostics are experiencing rapid development recently
due to the pressing need for the understanding of highly turbulent flows/flames that are …

Fluorescence is a ubiquitous readout of molecular localisation in the life sciences and has
enabled the elucidation of many biological processes, particularly since the development of …

Optical projection tomography (OPT) provides a non-invasive 3-D imaging modality that can
be applied to longitudinal studies of live disease models, including in zebrafish. Current …

We demonstrate the application of fluorescence lifetime optical projection tomography (FLIM-
OPT) to in vivo imaging of lysC: GFP transgenic zebrafish embryos (Danio rerio). This …

This review focuses on technical advances in fluorescence microscopy techniques including
laser scanning techniques, fluorescence‐resonance energy transfer (FRET) microscopy …

We describe a versatile optical projection tomography system for rapid three-dimensional
imaging of microscopic specimens in vivo. Our tomographic setup eliminates the in xy and z …