[HTML][HTML] Complex multimeric [FeFe] hydrogenases: biochemistry, physiology and new opportunities for the hydrogen economy

K Schuchmann, NP Chowdhury, V Müller - Frontiers in microbiology, 2018 - frontiersin.org
Hydrogenases are key enzymes of the energy metabolism of many microorganisms.
Especially in anoxic habitats where molecular hydrogen (H2) is an important intermediate …

[HTML][HTML] Formate hydrogenlyase, formic acid translocation and hydrogen production: Dynamic membrane biology during fermentation

K Peters, F Sargent - Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2023 - Elsevier
Abstract Formate hydrogenlyase-1 (FHL-1) is a complex-I-like enzyme that is commonly
found in gram-negative bacteria. The enzyme comprises a peripheral arm and a membrane …

Evidence for bidirectional formic acid translocation in vivo via the Escherichia coli formate channel FocA

L Vanyan, M Kammel, RG Sawers… - Archives of Biochemistry …, 2024 - Elsevier
Pentameric FocA permeates either formate or formic acid bidirectionally across the
cytoplasmic membrane of anaerobically growing Escherichia coli. Each protomer of FocA …

Regulation of metabolism and proton motive force generation during mixed carbon fermentation by an Escherichia coli strain lacking the FOF1-ATPase

H Gevorgyan, L Baghdasaryan, K Trchounian - Biochimica et Biophysica …, 2024 - Elsevier
Abstract Proton FOF 1-ATPase is the key enzyme in E. coli under fermentative conditions. In
this study the role of E. coli proton ATPase in the μ and formation of metabolic pathways …

HyfF subunit of hydrogenase 4 is crucial for regulating FOF1 dependent proton/potassium fluxes during fermentation of various concentrations of glucose

L Vanyan, K Trchounian - Journal of Bioenergetics and Biomembranes, 2022 - Springer
Escherichia coli anaerobically ferment glucose and perform proton/potassium exchange at
pH 7.5. The role of hyf (hydrogenase 4) subunits (HyfBDF) in sensing different …

[HTML][HTML] On-line analysis and in situ pH monitoring of mixed acid fermentation by Escherichia coli using combined FTIR and Raman techniques

GD Metcalfe, TW Smith, M Hippler - Analytical and Bioanalytical Chemistry, 2020 - Springer
We introduce an experimental setup allowing continuous monitoring of bacterial
fermentation processes by simultaneous optical density (OD) measurements, long-path FTIR …

[HTML][HTML] Osmotic stress as a factor for regulating E. coli hydrogenase activity and enhancing H2 production during mixed carbon sources fermentation

A Babayan, A Vassilian, K Trchounian - AIMS microbiology, 2023 - ncbi.nlm.nih.gov
Escherichia coli performs mixed-acid fermentation and produces molecular hydrogen (H 2)
via reversible hydrogenases (Hyd). H 2 producing activity was investigated during hyper …

Defining the roles of the hydrogenase 3 and 4 subunits in hydrogen production during glucose fermentation: a new model of a H2-producing hydrogenase complex

H Petrosyan, L Vanyan, A Trchounian… - International Journal of …, 2020 - Elsevier
Abstract Hydrogen (H 2)-producing hydrogenase (Hyd) activity of E. coli wild type and
mutants with defects in subunits of Hyd-3 or Hyd-4 during fermentation at different glucose …

The influence of hydrogen production on the formation of metabolic pathways and regulation of ΔpH in Escherichia coli

H Gevorgyan, A Vassilian, A Poladyan… - International Journal of …, 2022 - Elsevier
Hydrogen (H 2) metabolism in Escherichia coli occurs via reversible membrane-associated
hydrogenase enzymes (Hyd). Hyd-3 and Hyd-4 with formate dehydrogenase H (FDH-H) …

Identification Process and Physiological Properties of Transporters of Carboxylic Acids in Escherichia coli

S Kim, HK Lee, GY Jung - Biotechnology and Bioprocess Engineering, 2022 - Springer
Carboxylic acids (CAs) are important organic compounds that participate in various cellular
metabolic pathways. Since maintenance of intracellular CA concentration is vital for cell …