Activated charcoal is commonly used in tissue culture media. Its addition to culture medium
may promote or inhibit in vitro growth, depending on species and tissues used. The effects of …

Regeneration of plants by somatic embryogenesis (SE) was achieved in Stone pine (Pinus
pinea), one of the most characteristic tree species of the Mediterranean ecosystem. The …

An efficient microprogation protocol has been developed for Dendrobium densiflorum Lindl.
ex Wall., a traditional medicinal plant, through protocorm-like bodies (PLBs) from nodal stem …

This study is the first report of a protocol for transfer and expression of foreign chimeric
genes into cotyledons excised from Pinus pinea L. embryos. Agrobacterium tumefaciens …

The propagation of adult conifer trees by tissue culture has been studied for the last twenty
years, but problems related to the juvenile to adult phase change of trees have limited the …

A protocol for micropropagation from isolated cotyledons from Pinus pinea L. has been
developed. Major improvements are the increase in rooting rates and the description for the …

Isolated cotyledons from Pinus pinea L. germinated embryos cultured in vitro showed shoot
organogenesis in response to treatment with 4.4 μM N6-benzyladenine (BA). In late …

Isolated cotyledons from mature Pinus pinea L. embryos were cultured in vitro in a factorial
combination of 4.4, 10 and 44.4 μM N 6-benzyladenine (BA) for 2, 4, 8, 16 and 35 days to …

Plantlet regeneration was achieved in blue pine (Pinus wallichiana AB Jacks) by
organogenesis of mature zygotic embryos. The effect of various basal media and five …

Adventitious shoots were induced on cotyledons of Pinus pinea. Among seven salt
formulations, three carbon sources (sucrose, glucose and fructose), and two cytokinins [6 …