Exome sequencing: current and future perspectives

A Warr, C Robert, D Hume, A Archibald… - G3: Genes …, 2015 - academic.oup.com
The completion of a reference genome sequence for humans took more than 200 scientists
more than a decade in a project that cost almost $3 billion to complete (International Human …

Analysis of plant microbe interactions in the era of next generation sequencing technologies

C Knief - Frontiers in plant science, 2014 - frontiersin.org
Next generation sequencing (NGS) technologies have impressively accelerated research in
biological science during the last years by enabling the production of large volumes of …

proovread : large-scale high-accuracy PacBio correction through iterative short read consensus

T Hackl, R Hedrich, J Schultz, F Förster - Bioinformatics, 2014 - academic.oup.com
Motivation: Today, the base code of DNA is mostly determined through sequencing by
synthesis as provided by the Illumina sequencers. Although highly accurate, resulting reads …

Advantages of genome sequencing by long-read sequencer using SMRT technology in medical area

K Nakano, A Shiroma, M Shimoji, H Tamotsu… - Human cell, 2017 - Springer
PacBio RS II is the first commercialized third-generation DNA sequencer able to sequence a
single molecule DNA in real-time without amplification. PacBio RS II's sequencing …

In silico Screening and Heterologous Expression of a Polyethylene Terephthalate Hydrolase (PETase)-Like Enzyme (SM14est) With Polycaprolactone (PCL)-Degrading Activity …

EL Almeida, AF Carrillo Rincón, SA Jackson… - Frontiers in …, 2019 - frontiersin.org
Plastics, such as the polyethylene terephthalate (PET), are widely used for various industrial
applications, due to their physicochemical properties which are particularly useful in the …

Detailed analysis of HTT repeat elements in human blood using targeted amplification‐free long‐read sequencing

I Höijer, YC Tsai, TA Clark, P Kotturi, N Dahl… - Human …, 2018 - Wiley Online Library
Amplification of DNA is required as a mandatory step during library preparation in most
targeted sequencing protocols. This can be a critical limitation when targeting regions that …

Evaluation and validation of de novo and hybrid assembly techniques to derive high-quality genome sequences

SM Utturkar, DM Klingeman, ML Land… - …, 2014 - academic.oup.com
Motivation: To assess the potential of different types of sequence data combined with de
novo and hybrid assembly approaches to improve existing draft genome sequences …

Genome sequence of Plasmopara viticola and insight into the pathogenic mechanism

L Yin, Y An, J Qu, X Li, Y Zhang, I Dry, H Wu, J Lu - Scientific Reports, 2017 - nature.com
Plasmopara viticola causes downy mildew disease of grapevine which is one of the most
devastating diseases of viticulture worldwide. Here we report a 101.3 Mb whole genome …

Nematode mitochondrial metagenomics: A new tool for biodiversity analysis

EM Gendron, JL Sevigny, I Byiringiro… - Molecular Ecology …, 2023 - Wiley Online Library
DNA barcoding approaches have greatly increased our understanding of biodiversity on the
planet, and metabarcoding is widely used for classifying members of the phylum Nematoda …

Cell separations and sorting

MA Witek, IM Freed, SA Soper - Analytical chemistry, 2019 - ACS Publications
The development of new methods and technologies for cell separation, sorting, selection,
isolation, or enrichment (many times the aforementioned terms are used interchangeably) …