Challenges in the use of sortase and other peptide ligases for site-specific protein modification
HE Morgan, WB Turnbull, ME Webb - Chemical Society Reviews, 2022 - pubs.rsc.org
Site-specific protein modification is a widely-used biochemical tool. However, there are
many challenges associated with the development of protein modification techniques, in …
many challenges associated with the development of protein modification techniques, in …
Asparaginyl ligases: new enzymes for the protein engineer's toolbox
Enzyme‐catalysed site‐specific protein modifications enable the precision manufacture of
conjugates for the study of protein function and/or for therapeutic or diagnostic applications …
conjugates for the study of protein function and/or for therapeutic or diagnostic applications …
pH-controlled protein orthogonal ligation using asparaginyl peptide ligases
Peptide asparaginyl ligases (PALs) catalyze transpeptidation at the Asn residue of a short
Asn-Xaa1-Xaa2 tripeptide motif. Due to their high catalytic activity toward the P1-Asn …
Asn-Xaa1-Xaa2 tripeptide motif. Due to their high catalytic activity toward the P1-Asn …
Structure–function study of a Ca2+-independent metacaspase involved in lateral root emergence
Metacaspases are part of an evolutionarily broad family of multifunctional cysteine
proteases, involved in disease and normal development. As the structure–function …
proteases, involved in disease and normal development. As the structure–function …
Protein degradation by human 20S proteasomes elucidates the interplay between peptide hydrolysis and splicing
If and how proteasomes catalyze not only peptide hydrolysis but also peptide splicing is an
open question that has divided the scientific community. The debate has so far been based …
open question that has divided the scientific community. The debate has so far been based …
Structural basis for proenzyme maturation, substrate recognition, and ligation by a hyperactive peptide asparaginyl ligase
Peptide ligases are versatile enzymes that can be utilized for precise protein conjugation for
bioengineering applications. Hyperactive peptide asparaginyl ligases (PALs), such as …
bioengineering applications. Hyperactive peptide asparaginyl ligases (PALs), such as …
Phytocystatin 6 is a context‐dependent, tight‐binding inhibitor of Arabidopsis thaliana legumain isoform β
Plant legumains are crucial for processing seed storage proteins and are critical regulators
of plant programmed cell death. Although research on legumains boosted recently, little is …
of plant programmed cell death. Although research on legumains boosted recently, little is …
Substrate‐binding glycine residues are major determinants for hydrolase and ligase activity of plant legumains
Peptide asparaginyl ligases (PALs) are useful tools for precision modifications of proteins
and live‐cell surfaces by ligating peptides after Asn/Asp (Asx). They share high sequence …
and live‐cell surfaces by ligating peptides after Asn/Asp (Asx). They share high sequence …
[PDF][PDF] Structure-function study of a Ca2+-independent metacaspase involved in lateral root emergence.
Structure–function study of a Ca2+-independent metacaspase involved in lateral root
emergence Page 1 PNAS 2023 Vol. 120 No. 22 e2303480120 https://doi.org/10.1073/pnas.2303480120 …
emergence Page 1 PNAS 2023 Vol. 120 No. 22 e2303480120 https://doi.org/10.1073/pnas.2303480120 …
Legumain Activity Is Controlled by Extended Active Site Residues and Substrate Conformation
T Elamin, H Brandstetter, E Dall - International Journal of Molecular …, 2022 - mdpi.com
Legumain is a lysosomal cysteine protease with strict specificity for cleaving after
asparagine residues. By sequence comparison, legumain belongs to MEROPS clan CD of …
asparagine residues. By sequence comparison, legumain belongs to MEROPS clan CD of …