Redesigning of microbial cell surface and its application to whole-cell biocatalysis and biosensors

L Han, Y Zhao, S Cui, B Liang - Applied biochemistry and biotechnology, 2018 - Springer
Microbial cell surface display technology can redesign cell surfaces with functional proteins
and peptides to endow cells some unique features. Foreign peptides or proteins are …

Progress in research and application development of surface display technology using Bacillus subtilis spores

P Lin, H Yuan, J Du, K Liu, H Liu, T Wang - Applied microbiology and …, 2020 - Springer
Bacillus subtilis is a widely distributed aerobic Gram-positive species of bacteria. As a tool in
the lab, it has the advantages of nonpathogenicity and limited likelihood of becoming drug …

Bacterial spores as platforms for bioanalytical and biomedical applications

LD Knecht, P Pasini, S Daunert - Analytical and bioanalytical chemistry, 2011 - Springer
Genetically engineered bacteria-based sensing systems have been employed in a variety of
analyses because of their selectivity, sensitivity, and ease of use. These systems, however …

Proteins involved in formation of the outermost layer of Bacillus subtilis spores

D Imamura, R Kuwana, H Takamatsu… - Journal of …, 2011 - Am Soc Microbiol
To investigate the outermost structure of the Bacillus subtilis spore, we analyzed the
accessibility of antibodies to proteins on spores of B. subtilis. Anti-green fluorescent protein …

Spore surface display

R Isticato, E Ricca - The bacterial spore: from molecules to …, 2016 - Wiley Online Library
Display systems that present biologically active molecules on the surface of microorganisms
have become increasingly used to address environmental and biomedical issues (1–3) …

Expression and display of UreA of Helicobacter acinonychis on the surface of Bacillus subtilis spores

K Hinc, R Isticato, M Dembek, J Karczewska… - Microbial cell …, 2010 - Springer
Background The bacterial endospore (spore) has recently been proposed as a new surface
display system. Antigens and enzymes have been successfully exposed on the surface …

Recent progress in Bacillus subtilis spore-surface display: concept, progress, and future

H Wang, Y Wang, R Yang - Applied Microbiology and Biotechnology, 2017 - Springer
With the increased knowledge on spore structure and advances in biotechnology
engineering, the newly developed spore-surface display system confers several inherent …

Oral vaccine delivery by recombinant spore probiotics

SM Cutting, HA Hong, L Baccigalupi… - International reviews of …, 2009 - Taylor & Francis
Over the past few decades, advancements in molecular and cell biology have allowed
scientists to identify a large number of new antigens from a variety of viral and bacterial …

Expression and display of Clostridium difficile protein FliD on the surface of Bacillus subtilis spores

A Negri, W Potocki, A Iwanicki… - Journal of medical …, 2013 - microbiologyresearch.org
The endospores of Bacillus subtilis can serve as a tool for surface presentation of
heterologous proteins. The unique properties of the spore protective layers make them …

Nanoscale Chemical Imaging of Bacillus subtilis Spores by Combining Tip-Enhanced Raman Scattering and Advanced Statistical Tools

G Rusciano, G Zito, R Isticato, T Sirec, E Ricca… - Acs Nano, 2014 - ACS Publications
Tip-enhanced Raman Scattering (TERS) has recently emerged as a powerful spectroscopic
technique capable of providing subdiffraction morphological and chemical information on …