Importance of the 5′ regulatory region to bacterial synthetic biology applications

L Tietze, R Lale - Microbial Biotechnology, 2021 - Wiley Online Library
The field of synthetic biology is evolving at a fast pace. It is advancing beyond single‐gene
alterations in single hosts to the logical design of complex circuits and the development of …

mCherry contains a fluorescent protein isoform that interferes with its reporter function

M Fages-Lartaud, L Tietze, F Elie, R Lale… - … in Bioengineering and …, 2022 - frontiersin.org
Fluorescent proteins are essential reporters in cell and molecular biology. Here, we found
that red-fluorescent proteins possess an alternative translation initiation site that produces a …

[HTML][HTML] Engineering artificial cross-species promoters with different transcriptional strengths

W Zuo, G Yin, L Zhang, W Zhang, R Xu, Y Wang… - Synthetic and Systems …, 2025 - Elsevier
As a fundamental tool in synthetic biology, promoters are pivotal in regulating gene
expression, enabling precise genetic control and spurring innovation across diverse …

[HTML][HTML] Employing synthetic biology to expand antibiotic discovery

GD Cook, NM Stasulli - SLAS technology, 2024 - Elsevier
Antimicrobial-resistant (AMR) bacterial pathogens are a continually growing threat as our
methods for combating these infections continue to be overcome by the evolution of …

[HTML][HTML] Identification and cross-characterisation of artificial promoters and 5′ untranslated regions in Vibrio natriegens

L Tietze, A Mangold, MW Hoff, R Lale - Frontiers in Bioengineering …, 2022 - frontiersin.org
Vibrio natriegens has recently gained attention as a novel fast-growing bacterium in
synthetic biology applications. Currently, a limited set of genetic elements optimised for …

[PDF][PDF] Establishment of novel model systems for synthetic microbial communities

M Mager - 2024 - docserv.uni-duesseldorf.de
While most of microbiological research focuses on the study of individual species, research
on microbial consortia is still in its infancy. However, microbes in nature exist almost …

Standard Intein Gene Expression Ramps (SIGER) for protein-independent expression control

M Fages-Lartaud, Y Mueller, F Elie… - ACS Synthetic …, 2023 - ACS Publications
Coordination of multigene expression is one of the key challenges of metabolic engineering
for the development of cell factories. Constraints on translation initiation and early ribosome …

High-resolution mapping of Sigma Factor DNA Binding Sequences using Artificial Promoters, RNA Aptamers and Deep Sequencing

EA Khan, C Rückert-Reed, GS Dahiya, L Tietze… - bioRxiv, 2024 - biorxiv.org
The variable sigma (σ) subunit of the bacterial RNA polymerase holoenzyme determines
promoter specificity and facilitate open complex formation during transcription initiation …