High-throughput screening, next generation sequencing and machine learning: advanced methods in enzyme engineering

R Vanella, G Kovacevic, V Doffini… - Chemical …, 2022 - pubs.rsc.org
Enzyme engineering is an important biotechnological process capable of generating
tailored biocatalysts for applications in industrial chemical conversion and biopharma …

Antioxidant activity of synthetic polymers of phenolic compounds

S Nagarajan, R Nagarajan, J Kumar, A Salemme… - Polymers, 2020 - mdpi.com
In recent years, developing potent antioxidants has been a very active area of research. In
this context, phenolic compounds have been evaluated for their antioxidant activity …

Cytoprotection of human progenitor and stem cells through encapsulation in alginate templated, dual crosslinked silk and silk–gelatin composite hydrogel microbeads

O Hasturk, JA Smiley, M Arnett… - Advanced …, 2022 - Wiley Online Library
Susceptibility of mammalian cells against harsh processing conditions limit their use in cell
transplantation and tissue engineering applications. Besides modulation of the cell …

Cation-induced shape programming and morphing in protein-based hydrogels

LR Khoury, M Slawinski, DR Collison, I Popa - Science advances, 2020 - science.org
Smart materials that are capable of memorizing a temporary shape, and morph in response
to a stimulus, have the potential to revolutionize medicine and robotics. Here, we introduce …

Supramolecular liquid-crystalline polymer organogel: Fabrication, multiresponsiveness, and holographic switching properties

Y Ni, X Li, J Hu, S Huang, H Yu - Chemistry of Materials, 2019 - ACS Publications
Stimuli-responsive supramolecular organogels are fascinating for their dynamically
controllable features, but it is difficult for most versatile linear vinyl polymers to construct …

A simple hydrogel scaffold with injectability, adhesivity and osteogenic activity for bone regeneration

H Zhang, Q Cai, Y Zhu, W Zhu - Biomaterials Science, 2021 - pubs.rsc.org
Numerous efforts have been made to prepare hydrogels with injectability, adhesivity and
osteogenic activity for bone regeneration. However, current hydrogels with these …

Enzyme cascade with horseradish peroxidase readout for high-throughput screening and engineering of human Arginase-1

J Fernández De Santaella, J Ren, R Vanella… - Analytical …, 2023 - ACS Publications
We report an enzyme cascade with horseradish peroxidase-based readout for screening
human arginase-1 (hArg1) activity. We combined the four enzymes hArg1, ornithine …

Advanced strategies in high-throughput droplet screening for enzyme engineering

W Yan, X Li, D Zhao, M Xie, T Li, L Qian, C Ye… - Biosensors and …, 2023 - Elsevier
Enzymes, as biocatalysts, play a cumulatively important role in environmental purification
and industrial production of chemicals and pharmaceuticals. However, natural enzymes are …

Enzyme‐mediated hydrogel encapsulation of single cells for high‐throughput screening and directed evolution of oxidoreductases

R Vanella, DT Ta, MA Nash - Biotechnology and …, 2019 - Wiley Online Library
Directed evolution of oxidoreductases to improve their catalytic properties is being ardently
pursued in the industrial, biotechnological, and biopharma sectors. Hampering this pursuit …

Chemical structure–Antioxidant activity relationship of water–based enzymatic polymerized rutin and its wound healing potential

T Pivec, R Kargl, U Maver, M Bračič, T Elschner… - Polymers, 2019 - mdpi.com
The flavonoid rutin (RU) is a known antioxidant substance of plant origin. Its potential
application in pharmaceutical and cosmetic fields is, however, limited, due to its low water …