Selection of Nucleotide-Encoded Mass Libraries of Macrocyclic Peptides for Inaccessible Drug Targets

K Colas, D Bindl, H Suga - Chemical Reviews, 2024 - ACS Publications
Technological advances and breakthrough developments in the pharmaceutical field are
knocking at the door of the “undruggable” fortress with increasing insistence. Notably, the …

Engineering thermostability of industrial enzymes for enhanced application performance

K Xu, H Fu, Q Chen, R Sun, R Li, X Zhao, J Zhou… - International Journal of …, 2024 - Elsevier
Thermostability is a key factor for the industrial application of enzymes. This review
categorizes enzymes by their applications and discusses the importance of engineering …

Photonics of Hydrothermally Treated β‐Lactoglobulin Amyloids

P Hanczyc, SR Alfarano, S Bolisetty, J Zhou… - Small …, 2024 - Wiley Online Library
Increased temperature and high pressure are applied to β‐lactoglobulin fibrils in the
autoclave, resulting in the acquisition of a composite material comprised of partially …

Photonics of Hydrothermally Treated β-Lactoglobulin Amyloids

P Hanczyc, SR Alfarano, S Bolisetty… - Small …, 2024 - research-collection.ethz.ch
Increased temperature and high pressure are applied to beta-lactoglobulin fibrils in the
autoclave, resulting in the acquisition of a composite material comprised of partially …

Optimized single-cell gates for yeast display screening

X Pan, MO de Souza, FM Figueiras… - Protein Engineering …, 2025 - academic.oup.com
Yeast display is a widely used technology in antibody discovery and protein engineering.
The cell size of yeast enables fluorescence-activated cell sorting (FACS) to precisely screen …

Screening macrocyclic peptide libraries by yeast display allows control of selection process and affinity ranking

S Linciano, Y Mazzocato, Z Romanyuk, F Vascon… - bioRxiv, 2024 - biorxiv.org
Macrocyclic peptides provide an attractive modality for drug development due to their ability
to bind challenging targes, their small size, and amenability to powerful in vitro evolution …

High-throughput protein binder discovery by rapid in vivo selection

MJ Styles, JA Pixley, T Wei, C Basile, SS Lu… - bioRxiv, 2025 - biorxiv.org
Proteins that selectively bind to a target of interest are foundational components of research
pipelines,, diagnostics, and therapeutics. Current immunization-based,, display-based–, and …

Production, Characterization and Engineering of a Variable Lymphocyte Receptor that Targets the Extracellular Matrix of the Brain

EA Appelt - 2024 - search.proquest.com
Variable lymphocyte receptors (VLRs) are a type of antigen receptor derived from the
adaptive immune system of jawless vertebrates such as lamprey (Petromyzon marinus) …