Guidelines for the use of flow cytometry and cell sorting in immunological studies

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2019 - Wiley Online Library
These guidelines are a consensus work of a considerable number of members of the
immunology and flow cytometry community. They provide the theory and key practical …

Guidelines for the use of flow cytometry and cell sorting in immunological studies*

A Cossarizza, HD Chang, A Radbruch… - European journal of …, 2017 - Wiley Online Library
From a technical point of view a flow cytometer is a light detection device capable of
detecting photons of different wavelengths over a high dynamic range. In order to achieve a …

[HTML][HTML] Advancements in technology for characterizing the tumor immune microenvironment

H Yan, X Ju, A Huang, J Yuan - International Journal of Biological …, 2024 - ncbi.nlm.nih.gov
Immunotherapy plays a key role in cancer treatment, however, responses are limited to a
small number of patients. The biological basis for the success of immunotherapy is the …

Applications of single-cell omics in tumor immunology

J Liu, S Qu, T Zhang, Y Gao, H Shi, K Song… - Frontiers in …, 2021 - frontiersin.org
The tumor microenvironment (TME) is an ecosystem that contains various cell types,
including cancer cells, immune cells, stromal cells, and many others. In the TME, cancer …

ANPELA: Significantly Enhanced Quantification Tool for Cytometry‐Based Single‐Cell Proteomics

Y Zhang, H Sun, X Lian, J Tang, F Zhu - Advanced science, 2023 - Wiley Online Library
ANPELA is widely used for quantifying traditional bulk proteomic data. Recently, there is a
clear shift from bulk proteomics to the single‐cell ones (SCP), for which powerful cytometry …

Characterization of human monocyte subsets by whole blood flow cytometry analysis

R Marimuthu, H Francis, S Dervish… - Journal of …, 2018 - pmc.ncbi.nlm.nih.gov
Monocytes are key contributors in various inflammatory disorders and alterations to these
cells, including their subset proportions and functions, can have pathological significance …

Analysis of bacterial-surface-specific antibodies in body fluids using bacterial flow cytometry

K Moor, J Fadlallah, A Toska, D Sterlin, ML Balmer… - Nature protocols, 2016 - nature.com
Antibacterial antibody responses that target surfaces of live bacteria or secreted toxins are
likely to be relevant in controlling bacterial pathogenesis. The ability to specifically quantify …

A guide to flow cytometry: components, basic principles, experimental design, and cancer research applications

L El‐Hajjar, F Ali Ahmad, R Nasr - Current Protocols, 2023 - Wiley Online Library
Flow cytometry (FCM) is a state‐of‐the‐art technique for the qualitative and quantitative
assessment of cells and other particles' physical and biological properties. These cells are …

Detection of rare objects by flow cytometry: imaging, cell sorting, and deep learning approaches

DV Voronin, AA Kozlova, RA Verkhovskii… - International journal of …, 2020 - mdpi.com
Flow cytometry nowadays is among the main working instruments in modern biology paving
the way for clinics to provide early, quick, and reliable diagnostics of many blood-related …

Guidelines on antibody use in physiology research

HL Brooks, LE de Castro Brás… - American Journal …, 2024 - journals.physiology.org
Antibodies are one of the most used reagents in scientific laboratories and are critical
components for a multitude of experiments in physiology research. Over the past decade …