Although bacterial genomes are usually densely protein-coding, genome-wide mapping
approaches of transcriptional start sites revealed that a significant fraction of the identified …

Dynamic regulation is a promising strategy for fine-tuning metabolic fluxes in microbial cell
factories. However, few of these synthetic regulatory systems have been developed for …

In recent years, transcriptional roadblocking has emerged as a crucial regulatory
mechanism in gene expression, whereby other DNA-bound obstacles can block the …

Synthetic genetic circuits offer the potential to wield computational control over biology, but
their complexity is limited by the accuracy of mathematical models. Here, we present …

Cell differentiation programs require dynamic regulation of gene expression. During meiotic
prophase in Saccharomyces cerevisiae, expression of the kinetochore complex subunit …

DNA looping is a ubiquitous and critical feature of gene regulation. Although DNA looping
can be efficiently detected, tools to readily manipulate DNA looping are limited. Here we …

Genomic integration of heterologous genes is the preferred approach in industrial
fermentation-related strains due to the drawbacks associated with plasmid-mediated …

MCF7 cells acquire estrogen-independent proliferation after long-term estrogen deprivation
(LTED), which recapitulates endocrine therapy resistance. LTED cells can become primed …

Terpenoids are the largest family of natural products. They are made from the building block
isoprene pyrophosphate (IPP), and their bioproduction using engineered cell factories has …

SAM (S-adenosylmethionine) is an important metabolite that operates as a major donor of
methyl groups and is a controller of various physiological processes. Its availability is also …