Nanodiscs: A toolkit for membrane protein science
SG Sligar, IG Denisov - Protein Science, 2021 - Wiley Online Library
Membrane proteins are involved in numerous vital biological processes, including transport,
signal transduction and the enzymes in a variety of metabolic pathways. Integral membrane …
signal transduction and the enzymes in a variety of metabolic pathways. Integral membrane …
FRET-based dynamic structural biology: Challenges, perspectives and an appeal for open-science practices
Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …
Conformational activation promotes CRISPR-Cas12a catalysis and resetting of the endonuclease activity
Cas12a, also known as Cpf1, is a type VA CRISPR-Cas RNA-guided endonuclease that is
used for genome editing based on its ability to generate specific dsDNA breaks. Here, we …
used for genome editing based on its ability to generate specific dsDNA breaks. Here, we …
Biased cytochrome P450-mediated metabolism via small-molecule ligands binding P450 oxidoreductase
SB Jensen, S Thodberg, S Parween, ME Moses… - Nature …, 2021 - nature.com
Metabolic control is mediated by the dynamic assemblies and function of multiple redox
enzymes. A key element in these assemblies, the P450 oxidoreductase (POR), donates …
enzymes. A key element in these assemblies, the P450 oxidoreductase (POR), donates …
Dynamic metabolic solutions to the sessile life style of plants
Covering: up to 2018 Plants are sessile organisms. To compensate for not being able to
escape when challenged by unfavorable growth conditions, pests or herbivores, plants have …
escape when challenged by unfavorable growth conditions, pests or herbivores, plants have …
[HTML][HTML] Molecular mechanism of metabolic NAD (P) H-dependent electron-transfer systems: The role of redox cofactors
T Iyanagi - Biochimica et Biophysica Acta (BBA)-Bioenergetics, 2019 - Elsevier
Abstract NAD (P) H-dependent electron-transfer (ET) systems require three functional
components: a flavin-containing NAD (P) H-dehydrogenase, one-electron carrier and metal …
components: a flavin-containing NAD (P) H-dehydrogenase, one-electron carrier and metal …
Direct observation of Thermomyces lanuginosus lipase diffusional states by Single Particle Tracking and their remodeling by mutations and inhibition
Lipases are interfacially activated enzymes that catalyze the hydrolysis of ester bonds and
constitute prime candidates for industrial and biotechnological applications ranging from …
constitute prime candidates for industrial and biotechnological applications ranging from …
Fundamentals to function: Quantitative and scalable approaches for measuring protein stability
Folding a linear chain of amino acids into a three-dimensional protein is a complex physical
process that ultimately confers an impressive range of diverse functions. Although recent …
process that ultimately confers an impressive range of diverse functions. Although recent …
[HTML][HTML] Stabilization of dhurrin biosynthetic enzymes from Sorghum bicolor using a natural deep eutectic solvent
In recent years, ionic liquids and deep eutectic solvents (DESs) have gained increasing
attention due to their ability to extract and solubilize metabolites and biopolymers in …
attention due to their ability to extract and solubilize metabolites and biopolymers in …
Defining optimal electron transfer partners for light-driven cytochrome P450 reactions
SB Mellor, MH Vinde, AZ Nielsen, GT Hanke… - Metabolic …, 2019 - Elsevier
Plants and cyanobacteria are promising heterologous hosts for metabolic engineering, and
particularly suited for expression of cytochrome P450 (P450s), enzymes that catalyse key …
particularly suited for expression of cytochrome P450 (P450s), enzymes that catalyse key …