Calcium ions generate versatile intracellular signals that control key functions in all types of
neurons. Imaging calcium in neurons is particularly important because calcium signals exert …

With potential relevance for brain-mapping work, hydrogel-based structures can now be built
from within biological tissue to allow subsequent removal of lipids without mechanical …

The use of femtosecond laser pulses allows precise and thermal-damage-free removal of
material (ablation) with wide-ranging scientific,,,,, medical,,,,, and industrial applications …

Whole-brain mesoscale mapping in primates has been hindered by large brain sizes and
the relatively low throughput of available microscopy methods. Here, we present an …

The structure of axonal arbors controls how signals from individual neurons are routed
within the mammalian brain. However, the arbors of very few long-range projection neurons …

What is the nature of the vascular architecture in the cortex that allows the brain to meet the
energy demands of neuronal computations? We used high-throughput histology to …

Here we describe an automated method, named serial two-photon (STP) tomography, that
achieves high-throughput fluorescence imaging of mouse brains by integrating two-photon …

Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the
best noninvasive means of fluorescence microscopy in tissue explants and living animals …

Two-photon excitation of fluorescent proteins is an attractive approach for imaging living
systems. Today researchers are eager to know which proteins are the brightest and what the …

Advances in molecular biology, organic chemistry, and materials science have recently
created several new classes of fluorescent probes for imaging in cell biology. Here we …