The cell biologist's guide to super-resolution microscopy

G Jacquemet, AF Carisey, H Hamidi… - Journal of Cell …, 2020 - journals.biologists.com
Fluorescence microscopy has become a ubiquitous method to observe the location of
specific molecular components within cells. However, the resolution of light microscopy is …

Expansion microscopy: super-resolution imaging with hydrogels

S Truckenbrodt - Analytical Chemistry, 2023 - ACS Publications
Guidelines, Not Rules 14 Chemical Principles of Accessing Biomolecules in Expansion
Microscopy 14 Accessing Proteins 14 Protein Anchoring with Indirect Readout 14 Protein …

Light microscopy of proteins in their ultrastructural context

O M'Saad, J Bewersdorf - Nature communications, 2020 - nature.com
Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of
the cell is a major challenge in fluorescence microscopy. We report the discovery of a new …

Ultrafast distant wound response is essential for whole-body regeneration

Y Fan, C Chai, P Li, X Zou, JE Ferrell, B Wang - Cell, 2023 - cell.com
Injury induces systemic responses, but their functions remain elusive. Mechanisms that can
rapidly synchronize wound responses through long distances are also mostly unknown …

A versatile tiling light sheet microscope for imaging of cleared tissues

Y Chen, X Li, D Zhang, C Wang, R Feng, X Li, Y Wen… - Cell Reports, 2020 - cell.com
We present a tiling light sheet microscope compatible with all tissue clearing methods for
rapid multicolor 3D imaging of cleared tissues with micron-scale (4× 4× 10 μm 3) to …

Ultrastructure expansion microscopy reveals the cellular architecture of budding and fission yeast

K Hinterndorfer, MH Laporte, F Mikus… - Journal of Cell …, 2022 - journals.biologists.com
The budding and fission yeasts Saccharomyces cerevisiae and Schizosaccharomyces
pombe have served as invaluable model organisms to study conserved fundamental cellular …

Nanoscale imaging of bacterial infections by sphingolipid expansion microscopy

R Götz, TC Kunz, J Fink, F Solger, J Schlegel… - Nature …, 2020 - nature.com
Expansion microscopy (ExM) enables super-resolution imaging of proteins and nucleic
acids on conventional microscopes. However, imaging of details of the organization of lipid …

MicroMagnify: a multiplexed expansion microscopy method for pathogens and infected tissues

Z Cheng, C Stefani, T Skillman, A Klimas… - Advanced …, 2023 - Wiley Online Library
Super‐resolution optical imaging tools are crucial in microbiology to understand the
complex structures and behavior of microorganisms such as bacteria, fungi, and viruses …

Subdiffraction-resolution fluorescence imaging of immunological synapse formation between NK cells and A. fumigatus by expansion microscopy

N Trinks, S Reinhard, M Drobny, L Heilig… - Communications …, 2021 - nature.com
Expansion microscopy (ExM) enables super-resolution fluorescence imaging on standard
microscopes by physical expansion of the sample. However, the investigation of interactions …

Direct visualization of living bacterial genotypes using CRISPR/Cas12a-circular reporter nanoprobes

Y Li, Y Pei, D Huang, R Xu, Y Wu, Y Li, R Zhou… - Biosensors and …, 2022 - Elsevier
Bacterial genotyping is important for understanding the complex microbiota. Although
fluorescence in situ hybridization (FISH) has enabled bacterial community identification with …