[HTML][HTML] Manufacturing challenges and rational formulation development for AAV viral vectors

A Srivastava, KMG Mallela, N Deorkar… - Journal of pharmaceutical …, 2021 - Elsevier
Adeno-associated virus (AAV) has emerged as a leading platform for gene delivery for
treating various diseases due to its excellent safety profile and efficient transduction to …

Current challenges in biotherapeutic particles manufacturing

MG Moleirinho, RJS Silva, PM Alves… - Expert opinion on …, 2020 - Taylor & Francis
Introduction: The development of novel complex biotherapeutics led to new challenges in
biopharmaceutical industry. The potential of these particles has been demonstrated by the …

Synthetic biology design as a paradigm shift toward manufacturing affordable adeno-associated virus gene therapies

LT Collins, S Ponnazhagan, DT Curiel - ACS synthetic biology, 2023 - ACS Publications
Gene therapy has demonstrated enormous potential for changing how we combat disease.
By directly engineering the genetic composition of cells, it provides a broad range of options …

[HTML][HTML] Anion exchange chromatography-based platform for the scalable purification of extracellular vesicles derived from human mesenchymal stromal cells

RM Silva, SS Rosa, R Cunha, CL da Silva… - Separation and …, 2023 - Elsevier
Extracellular vesicles (EVs) have emerged as a novel cell-free strategy with broad
biomedical applications in disease diagnosis and prognosis as well as therapy. Moving EVs …

Adenoviral vector COVID-19 vaccines: process and cost analysis

RG Ferreira, NF Gordon, R Stock, D Petrides - Processes, 2021 - mdpi.com
The COVID-19 pandemic has motivated the rapid development of numerous vaccines that
have proven effective against SARS-CoV-2. Several of these successful vaccines are based …

[HTML][HTML] 3D-printed ordered bed structures for chromatographic purification of enveloped and non-enveloped viral particles

MG Moleirinho, S Feast, AS Moreira, RJS Silva… - Separation and …, 2021 - Elsevier
In the last few decades we have witnessed a rapid advance in the design of new
biotherapeutic products using viral particles. The current limitations of manufacturing these …

Photoactivatable Heptamethine-based carbonic anhydrase inhibitors leading to New Anti-antibacterial agents

S Carradori, A Angeli, PS Sfragano, X Yzeiri… - International Journal of …, 2023 - mdpi.com
With the aim to propose innovative antimicrobial agents able to not only selectively inhibit
bacterial carbonic anhydrases (CAs) but also to be photoactivated by specific wavelengths …

Downstream process design for Gag HIV‐1 based virus‐like particles

E Lorenzo, L Miranda, F Gòdia… - Biotechnology and …, 2023 - Wiley Online Library
Virus‐like particles‐based vaccines have been gaining interest in recent years. The
manufacturing of these particles includes their production by cell culture followed by their …

Development of a novel purification method for AAV vectors using tangential flow filtration

R Miyaoka, Y Tsunekawa, Y Kurosawa… - Biotechnology and …, 2023 - Wiley Online Library
Adeno‐associated virus (AAV) vector can efficiently transduce therapeutic genes in various
tissue types with less side effects; however, owing to complex multistep processes during …

Process design and comparison for batch and continuous manufacturing of recombinant adeno-associated virus

O Yang, Y Tao, M Qadan, M Ierapetritou - Journal of Pharmaceutical …, 2023 - Springer
Recombinant adeno-associated virus (rAAV) is one of the most widely used viral vectors that
has been used for gene therapy to treat a variety of human diseases. As a newly developed …