Configured for the human gut microbiota: molecular mechanisms of dietary β-glucan utilization

B Golisch, Z Lei, K Tamura, H Brumer - ACS Chemical Biology, 2021 - ACS Publications
The β-glucans are a disparate group of structurally diverse polysaccharides, whose
members are widespread in human diets as components of the cell walls of plants, algae …

[HTML][HTML] Phosphorylase-catalyzed bottom-up synthesis of short-chain soluble cello-oligosaccharides and property-tunable cellulosic materials

B Nidetzky, C Zhong - Biotechnology Advances, 2021 - Elsevier
Cellulose-based materials are produced industrially in countless varieties via top-down
processing of natural lignocellulose substrates. By contrast, cellulosic materials are only …

Structure-function analysis of the cyclic β-1,2-glucan synthase from Agrobacterium tumefaciens

J Sedzicki, D Ni, F Lehmann, H Stahlberg… - Nature …, 2024 - nature.com
The synthesis of complex sugars is a key aspect of microbial biology. Cyclic β-1, 2-glucan
(CβG) is a circular polysaccharide critical for host interactions of many bacteria, including …

Functional and structural analysis of a cyclization domain in a cyclic β-1, 2-glucan synthase

N Tanaka, R Saito, K Kobayashi, H Nakai… - Applied Microbiology …, 2024 - Springer
Abstract Cyclic β-1, 2-glucan synthase (CGS) is a key enzyme in production of cyclic β-1, 2-
glucans (CβGs) which are involved in bacterial infection or symbiosis to host organisms …

β-1, 2-Glucans and associated enzymes

M Nakajima - Biologia, 2023 - Springer
Sugar chains are biopolymers that play various important roles for organisms, and their
structures are complex and diverse. Enzymes acting on sugar chains have undergone a …

[HTML][HTML] Cellodextrin phosphorylase from Ruminiclostridium thermocellum: X-ray crystal structure and substrate specificity analysis

EC O'Neill, G Pergolizzi, CEM Stevenson… - Carbohydrate …, 2017 - Elsevier
The GH94 glycoside hydrolase cellodextrin phosphorylase (CDP, EC 2.4. 1.49) produces
cellodextrin oligomers from short β-1→ 4-glucans and α-D-glucose 1-phosphate. Compared …

Engineering of cellobiose phosphorylase for the defined synthesis of cellotriose

Z Ubiparip, DS Moreno, K Beerens… - Applied Microbiology and …, 2020 - Springer
Cellodextrins are non-digestible oligosaccharides that have attracted interest from the food
industry as potential prebiotics. They are typically produced through the partial hydrolysis of …

[HTML][HTML] Disaccharide phosphorylases: structure, catalytic mechanisms and directed evolution

S Sun, C You - Synthetic and Systems Biotechnology, 2021 - Elsevier
Disaccharide phosphorylases (DSPs) are carbohydrate-active enzymes with outstanding
potential for the biocatalytic conversion of common table sugar into products with attractive …

Effects of dietary rumen–protected Lys levels on rumen fermentation and bacterial community composition in Holstein heifers

F Kong, Y Gao, M Tang, T Fu, Q Diao, Y Bi… - Applied Microbiology and …, 2020 - Springer
This study aimed to evaluate the effects of partial reducing rumen-protected Lys (RPLys) on
rumen fermentation and microbial composition in heifers. Three ruminal fistulated Holstein …

Discovery and biotechnological exploitation of glycoside-phosphorylases

A Li, M Benkoulouche, S Ladeveze, J Durand… - International Journal of …, 2022 - mdpi.com
Among carbohydrate active enzymes, glycoside phosphorylases (GPs) are valuable
catalysts for white biotechnologies, due to their exquisite capacity to efficiently re-modulate …