The last few years has seen rapid advances in the solid phase synthesis of oligonucleotides
(reviewed by Itakura et al., 1984; Gait, 1984; Caruthers, 1985) which combined with the …

Mutagenesis of proteins is of paramount importance chain reaction (PCR) or non-PCR, has
revolutionized the means by which mutants are obtained (2, 3). Mutafor understanding the …

Course Description The course enables students to gain basic skills in molecular diagnosis
and karyotyping. This includes: DNA isolation and measurements, electrophoresis of nucleic …

We have developed a new expression vector which allows efficient selection for
transfectants that express foreign genes at high levels. The vector is composed of a …

Polymerase chain reaction conditions were established for the in vitro amplification of
eukaryotic small subunit ribosomal (16S-like) rRNA genes. Coding regions from algae …

Three kinds of improvements have been introduced into the M13-based cloning systems.(1)
New Escherichia coli host strains have been constructed for the E. coli bacteriophage M13 …

With the polymerase chain reaction (PCR) and versatile primers that amplify the whole
cytochrome b gene (∼ 1140 bp), we obtained 17 complete gene sequences representing …

Reliable recovery of the 5′ region of the cytochrome c oxidase 1 (COI) gene is critical for
the ongoing effort to gather DNA barcodes for all fish species. In this study, we develop and …

Unique DNA sequences can be determined directly from mouse genomic DNA. A
denaturing gel separates by size mixtures of unlabeled DNA fragments from complete …

In this study, we demonstrate that the CXC family of chemokines displays disparate
angiogenic activity depending upon the presence or absence of the ELR motif. CXC …