An update on current and novel molecular diagnostics for the diagnosis of invasive fungal infections

JD Jenks, PL White, SE Kidd, T Goshia… - Expert Review of …, 2023 - Taylor & Francis
Background Invasive fungal infections cause millions of infections annually, but diagnosis
remains challenging. There is an increased need for low-cost, easy to use, highly sensitive …

Universal digital high-resolution melting for the detection of pulmonary mold infections

T Goshia, A Aralar, N Wiederhold… - Journal of Clinical …, 2024 - Am Soc Microbiol
Invasive mold infections (IMIs) are associated with high morbidity, particularly in
immunocompromised patients, with mortality rates between 40% and 80%. Early initiation of …

Universal digital high-resolution melt analysis for the diagnosis of bacteremia

A Aralar, T Goshia, N Ramchandar… - The Journal of Molecular …, 2024 - Elsevier
Fast and accurate diagnosis of bloodstream infection is necessary to inform treatment
decisions for septic patients, who face hourly increases in mortality risk. Blood culture …

Droplet digital PCR for quantification of PML-RARα in acute promyelocytic leukemia: a comprehensive comparison with real-time PCR

D Yuan, M Cui, S Yu, H Wang, R Jing - Analytical and bioanalytical …, 2019 - Springer
Real-time quantitative PCR (qPCR) has been widely implemented for molecular testing, but
there are still some inherent limitations that hamper its usefulness. Droplet digital PCR …

Improving quantitative power in digital PCR through digital high-resolution melting

A Aralar, Y Yuan, K Chen, Y Geng… - Journal of Clinical …, 2020 - Am Soc Microbiol
Applying digital PCR (dPCR) technology to challenging clinical and industrial detection
tasks has become more prevalent because of its capability for absolute quantification and …

Data-driven noise modeling of digital DNA melting analysis enables prediction of sequence discriminating power

L Langouche, A Aralar, M Sinha, SM Lawrence… - …, 2020 - academic.oup.com
Motivation The need to rapidly screen complex samples for a wide range of nucleic acid
targets, like infectious diseases, remains unmet. Digital High-Resolution Melt (dHRM) is an …

Improving bacteria identification from digital melt assay via oligonucleotide-based temperature calibration

A Traylor, PW Lee, K Hsieh, TH Wang - Analytica Chimica Acta, 2024 - Elsevier
Background Bacterial infections, especially polymicrobial infections, remain a threat to
global health and require advances in diagnostic technologies for timely and accurate …

Machine learning based DNA melt curve profiling enables automated novel genotype detection

A Boussina, L Langouche, AC Obirieze, M Sinha… - BMC …, 2024 - Springer
Surveillance for genetic variation of microbial pathogens, both within and among species,
plays an important role in informing research, diagnostic, prevention, and treatment activities …

[HTML][HTML] In-House Validation of Four Duplex Droplet Digital PCR Assays to Quantify GM Soybean Events

D Verginelli, S Ciuffa, K Spinella, D La Rocca, M Misto… - Foods, 2024 - mdpi.com
Due to the increasing number of authorized events in the European Union, it is crucial for the
official laboratories to enforce market control to detect and quantify genetically modified …

[HTML][HTML] Universal Digital High Resolution Melt for the detection of pulmonary mold infections

T Goshia, A Aralar, N Wiederhold, JD Jenks, SR Mehta… - bioRxiv, 2023 - ncbi.nlm.nih.gov
Background: Invasive mold infections (IMIs) such as aspergillosis, mucormycosis, fusariosis,
and lomentosporiosis are associated with high morbidity and mortality, particularly in …