Agrobacterium-mediated transformation as a tool for functional genomics in fungi

CB Michielse, PJJ Hooykaas, CA van den Hondel… - Current genetics, 2005 - Springer
In the era of functional genomics, the need for tools to perform large-scale targeted and
random mutagenesis is increasing. A potential tool is Agrobacterium-mediated fungal …

Unintended effects and their detection in genetically modified crops

F Cellini, A Chesson, I Colquhoun, A Constable… - Food and Chemical …, 2004 - Elsevier
The commercialisation of GM crops in Europe is practically non-existent at the present time.
The European Commission has instigated changes to the regulatory process to address the …

Abundance, distribution, and transcriptional activity of repetitive elements in the maize genome

BC Meyers, SV Tingey, M Morgante - Genome Research, 2001 - genome.cshlp.org
Long terminal repeat (LTR) retrotransposons have been shown to make up much of the
maize genome. Although these elements are known to be prevalent in plant genomes of a …

Transgene integration, organization and interaction in plants

A Kohli, RM Twyman, R Abranches, E Wegel… - Plant molecular …, 2003 - Springer
It has been appreciated for many years that the structure of a transgene locus can have a
major influence on the level and stability of transgene expression. Until recently, however, it …

Contribution of the Tos17 retrotransposon to rice functional genomics

H Hirochika - Current opinion in plant biology, 2001 - Elsevier
The ongoing international efforts of the Rice Genomic Sequencing Project have already
generated a large amount of sequence data. The next important challenge will be to …

Recent advances in rice biotechnology—towards genetically superior transgenic rice

S Bajaj, A Mohanty - Plant Biotechnology Journal, 2005 - Wiley Online Library
Rice biotechnology has made rapid advances since the first transgenic rice plants were
produced 15 years ago. Over the past decade, this progress has resulted in the …

Genetic transformation of barley (Hordeum vulgare L.) via infection of androgenetic pollen cultures with Agrobacterium tumefaciens

J Kumlehn, L Serazetdinova, G Hensel… - Plant Biotechnology …, 2006 - Wiley Online Library
A novel genetic transformation method for barley (Hordeum vulgare L.), based on infection
of androgenetic pollen cultures with Agrobacterium tumefaciens, is presented. Winter‐type …

High throughput T‐DNA insertion mutagenesis in rice: a first step towards in silico reverse genetics

C Sallaud, C Gay, P Larmande, M Bès… - The Plant …, 2004 - Wiley Online Library
Summary A library of 29 482 T‐DNA enhancer trap lines has been generated in rice cv.
Nipponbare. The regions flanking the T‐DNA left border from the first 12 707 primary …

Functional Genomics of Eukaryotic Photosynthesis Using Insertional Mutagenesis of Chlamydomonas reinhardtii

RM Dent, CM Haglund, BL Chin, MC Kobayashi… - Plant …, 2005 - academic.oup.com
The unicellular green alga Chlamydomonas reinhardtii is a widely used model organism for
studies of oxygenic photosynthesis in eukaryotes. Here we describe the development of a …

A genomics approach towards salt stress tolerance

HJ Bohnert, P Ayoubi, C Borchert, RA Bressan… - Plant physiology and …, 2001 - Elsevier
Abiotic stresses reduce plant productivity. We focus on gene expression analysis following
exposure of plants to high salinity, using salt-shock experiments to mimic stresses that affect …